LOCUS       sequence-91495-e        1686 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Nuclear zinc-binding FRET biosensor.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1686)
  AUTHORS   Miranda JG, Weaver AL, Qin Y, Park JG, Stoddard CI, Lin MZ, Palmer 
            AE
  TITLE     New alternately colored FRET sensors for simultaneous monitoring of 
            Zn(2)(+) in multiple cellular locations.
  JOURNAL   PLoS One. 2012;7(11):e49371. doi: 10.1371/journal.pone.0049371. Epub
            2012 Nov 16.
  PUBMED    23173058
REFERENCE   2  (bases 1 to 1686)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2012"; volume: "7(11):e49371. doi"; pages: " 
            10.1371/journal.pone.0049371. Epub 2012 Nov 16"
COMMENT     Sequence Label: pcDNA-NLS-ZapCmR1.1
FEATURES             Location/Qualifiers
     source          1..1686
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             4..24
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(88..109)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     696..717
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             973..1683
                     /codon_start=1
                     /product="monomeric red fluorescent protein derived from 
                     mRuby, with improved photophysical properties (Lam et al., 
                     2012)"
                     /label=mRuby2
                     /translation="MVSKGEELIKENMRMKVVMEGSVNGHQFKCTGEGEGNPYMGTQTM
                     RITVIEGGPLPFAFDILATSFMYGSRTFIKYPKGIPDFFKQSFPEGFTWERVTRYEDGG
                     VVTVMQDTSLEDGCLVYHVQVRGVNFPSNGPVMQKKTKGWEPNTEMMYPADGGLRGYTH
                     MALKVDGGGHLSCSFVTTYRSKKTVGNIKMPGIHAVDHRLERLEESDNEMFVVQREVAV
                     AKFAGLGGGMDELYK"
ORIGIN
        1 atgcctaaaa aaaaacgtaa agttgaagat gcttcggatc cgatggtgag caagggcgag
       61 gagctgttca ccggggtggt gcccatcctg gtcgagctgg acggcgacgt aaacggccac
      121 aagttcagcg tccgcggcga gggcgagggc gatgccacca acggcaagct gaccctgaag
      181 ttcatctgca ccaccggcaa gctgcccgtg ccctggccca ccctcgtgac caccttcggc
      241 tacggcgtgg cctgcttcag ccgctacccc gaccacatga agcagcacga cttcttcaag
      301 tccgccatgc ccgaaggcta cgtccaggag cgcaccatct ctttcaagga cgacggtacc
      361 tacaagaccc gcgccgaggt gaagttcgag ggcgacaccc tggtgaaccg catcgagctg
      421 aagggcatcg acttcaagga ggacggcaac atcctggggc acaagctgga gtacaacttc
      481 aacagccaca acgtctatat cacggccgac aagcagaaga acggcatcaa ggctaacttc
      541 aagatccgcc acaacgttga ggacggcagc gtgcagctcg ccgaccacta ccagcagaac
      601 acccccatcg gcgacggccc cgtgctgctg cccgacaacc actacctgag ccatcagtcc
      661 gccctgagca aagaccccaa cgagaagcgc gatcacatgg tcctgctgga gttcgtgacc
      721 gccgcccgca tgcataaaaa caatgactta aaacacaaat ggaaagaatg tcctgagtct
      781 tgtagctcac tatttgacct acaaagacat cttttgaagg atcatgtctc tcaagatttc
      841 aaacacccta tggaaccatt agcttgtaat tgggaggact gtgatttcct tggagatgat
      901 acatgttcca tagtgaacca tattaattgt caacatggta tcgagctcat ggtgtctaag
      961 ggcgaagagc tcatggtgtc taagggcgaa gagctgatca aggaaaatat gcgtatgaag
     1021 gtggtcatgg aaggttcggt caacggccac caattcaaat gcacaggtga aggagaaggc
     1081 aatccgtaca tgggaactca aaccatgagg atcacagtca tcgagggagg acccctgcca
     1141 tttgcctttg acattcttgc cacgtcgttc atgtatggca gccgtacttt tatcaagtac
     1201 ccgaaaggca ttcctgattt ctttaaacag tcctttcctg agggttttac ttgggaaaga
     1261 gttacgagat acgaagatgg tggagtcgtc accgtcatgc aggacaccag ccttgaggat
     1321 ggctgtctcg tttaccacgt ccaagtcaga ggggtaaact ttccctccaa tggtcccgtg
     1381 atgcagaaga agaccaaggg ttgggagcct aatacagaga tgatgtatcc agcagatggt
     1441 ggtctgaggg gatacactca tatggcactg aaagttgatg gtggtggcca tctgtcttgc
     1501 tctttcgtaa caacttacag gtcaaaaaag accgtcggga acatcaagat gcccggtatc
     1561 catgccgttg atcaccgcct ggaaaggtta gaggaaagtg acaatgaaat gttcgtagta
     1621 caacgcgaag tcgcagttgc caagttcgcc gggcttggtg gtgggatgga cgagctgtac
     1681 aagtaa
//
