LOCUS       sequence-91502-e         892 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Luciferase reporter assay for CIP2A with point mutation on miR-375 
            binding site.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 892)
  AUTHORS   Jung HM, Patel RS, Phillips BL, Wang H, Cohen DM, Reinhold WC, Chang
            LJ, Yang LJ, Chan EK
  TITLE     Tumor suppressor miR-375 regulates MYC expression via repression of 
            CIP2A coding sequence through multiple miRNA-mRNA interactions.
  JOURNAL   Mol Biol Cell. 2013 Jun;24(11):1638-48, S1-7. doi: 
            10.1091/mbc.E12-12-0891. Epub 2013 Apr 3.
  PUBMED    23552692
REFERENCE   2  (bases 1 to 892)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1091/mbc.E12-12-0891"; journalName: "Mol Biol Cell"; date: 
            "2013-06"; volume: "24"; issue: "11"; pages: "1638-48, S1-7"
COMMENT     Sequence Label: pMiR-CIP2A-miR-375-mutE
FEATURES             Location/Qualifiers
     source          1..892
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     40..56
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     40..56
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             complement(76..99)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             complement(118..147)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             complement(159..851)
                     /codon_start=1
                     /product="red fluorescent protein from Entacmaea 
                     quadricolor"
                     /label=TurboRFP
                     /note="mammalian codon-optimized"
                     /translation="MSELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTMKIKV
                     VEGGPLPFAFDILATSFMYGSKAFINHTQGIPDFFKQSFPEGFTWERITTYEDGGVLTA
                     TQDTSFQNGCIIYNVKINGVNFPSNGPVMQKKTRGWEANTEMLYPADGGLRGHSQMALK
                     LVGGGYLHCSFKTTYRSKKPAKNLKMPGFHFVDHRLERIKEADKETYVEQHEMAVAKYC
                     DLPSKLGHR"
ORIGIN
        1 actggggagg ggtcacaggg atgccacccg ggatctgttc aggaaacagc tatgaccgcg
       61 gccggccgtt taaaccttat cgtcgtcatc cttgtaatcc aggatatcat ttgctgccag
      121 atcctcttct gagatgagtt tctgctcgag ggacgcgatc tgtgccccag tttgctaggg
      181 aggtcgcagt acttggccac agccatctcg tgctgctcga cgtaggtctc tttgtcggcc
      241 tccttgattc tttccagtct gtggtccacg aagtggaagc cgggcatctt gaggttctta
      301 gcgggtttct tggatctgta tgtggtcttg aaggagcagt gcaggtagcc cccgcccacg
      361 agcttcaggg ccatctggct gtggcctctc aggccgccgt cagcggggta cagcatctcg
      421 gtgttggcct cccagccgcg tgttttcttc tgcatcacag ggccgttgga tgggaagttc
      481 accccgttga tcttgacgtt gtagatgatg cagccgttct ggaagctggt gtcctgggta
      541 gcggtcagca cgcccccgtc ttcgtatgtg gtgattctct cccatgtgaa gccctcaggg
      601 aaggactgct taaagaagtc ggggatgccc tgggtgtggt tgatgaaggc tttgctgccg
      661 tacatgaagc tggtagccag gatgtcgaag gcgaagggga gagggccgcc ctcgaccacc
      721 ttgatcttca tggtctgggt gccctcgtag ggcttgcctt cgccctcgga tgtgcacttg
      781 aagtggtggt tgttcacggt gccctccatg tacagcttca tgtgcatgtt ctccttgatc
      841 agctcgctca tggcgaacgc ggcgcagatc tcctcggtac tggatccagt cg
//
