LOCUS       sequence-91505-e        1689 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Cytosolic zinc-binding FRET biosensor.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1689)
  AUTHORS   Miranda JG, Weaver AL, Qin Y, Park JG, Stoddard CI, Lin MZ, Palmer 
            AE
  TITLE     New alternately colored FRET sensors for simultaneous monitoring of 
            Zn(2)(+) in multiple cellular locations.
  JOURNAL   PLoS One. 2012;7(11):e49371. doi: 10.1371/journal.pone.0049371. Epub
            2012 Nov 16.
  PUBMED    23173058
REFERENCE   2  (bases 1 to 1689)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2012"; volume: "7(11):e49371. doi"; pages: " 
            10.1371/journal.pone.0049371. Epub 2012 Nov 16"
COMMENT     Sequence Label: pcDNA-NES-ZapCmR2
FEATURES             Location/Qualifiers
     source          1..1689
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             10..36
                     /codon_start=1
                     /product="nuclear export signal from the HIV Rev protein 
                     (Fischer et al., 1995)"
                     /label=NES
                     /translation="LPPLERLTL"
     primer_bind     complement(91..112)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     699..720
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             976..1686
                     /codon_start=1
                     /product="monomeric red fluorescent protein derived from 
                     mRuby, with improved photophysical properties (Lam et al., 
                     2012)"
                     /label=mRuby2
                     /translation="MVSKGEELIKENMRMKVVMEGSVNGHQFKCTGEGEGNPYMGTQTM
                     RITVIEGGPLPFAFDILATSFMYGSRTFIKYPKGIPDFFKQSFPEGFTWERVTRYEDGG
                     VVTVMQDTSLEDGCLVYHVQVRGVNFPSNGPVMQKKTKGWEPNTEMMYPADGGLRGYTH
                     MALKVDGGGHLSCSFVTTYRSKKTVGNIKMPGIHAVDHRLERLEESDNEMFVVQREVAV
                     AKFAGLGGGMDELYK"
ORIGIN
        1 atgcttcaac ttcctcctct tgaacgtctt actctttcgg atccgatggt gagcaagggc
       61 gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc
      121 cacaagttca gcgtccgcgg cgagggcgag ggcgatgcca ccaacggcaa gctgaccctg
      181 aagttcatct gcaccaccgg caagctgccc gtgccctggc ccaccctcgt gaccaccttc
      241 ggctacggcg tggcctgctt cagccgctac cccgaccaca tgaagcagca cgacttcttc
      301 aagtccgcca tgcccgaagg ctacgtccag gagcgcacca tctctttcaa ggacgacggt
      361 acctacaaga cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag
      421 ctgaagggca tcgacttcaa ggaggacggc aacatcctgg ggcacaagct ggagtacaac
      481 ttcaacagcc acaacgtcta tatcacggcc gacaagcaga agaacggcat caaggctaac
      541 ttcaagatcc gccacaacgt tgaggacggc agcgtgcagc tcgccgacca ctaccagcag
      601 aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca accactacct gagccatcag
      661 tccgccctga gcaaagaccc caacgagaag cgcgatcaca tggtcctgct ggagttcgtg
      721 accgccgccc gcatgcataa aaacaatgac ttaaaacaca aatggaaaga atgtcctgag
      781 tcttgtagct cactatttga cctacaaaga catcttttga aggatcatgt ctctcaagat
      841 ttcaaacacc ctatggaacc attagctcat aattgggagg actgtgattt ccttggagat
      901 gatacatgtt ccatagtgaa ccatattaat tgtcaacatg gtatcgagct catggtgtct
      961 aagggcgaag agctcatggt gtctaagggc gaagagctga tcaaggaaaa tatgcgtatg
     1021 aaggtggtca tggaaggttc ggtcaacggc caccaattca aatgcacagg tgaaggagaa
     1081 ggcaatccgt acatgggaac tcaaaccatg aggatcacag tcatcgaggg aggacccctg
     1141 ccatttgcct ttgacattct tgccacgtcg ttcatgtatg gcagccgtac ttttatcaag
     1201 tacccgaaag gcattcctga tttctttaaa cagtcctttc ctgagggttt tacttgggaa
     1261 agagttacga gatacgaaga tggtggagtc gtcaccgtca tgcaggacac cagccttgag
     1321 gatggctgtc tcgtttacca cgtccaagtc agaggggtaa actttccctc caatggtccc
     1381 gtgatgcaga agaagaccaa gggttgggag cctaatacag agatgatgta tccagcagat
     1441 ggtggtctga ggggatacac tcatatggca ctgaaagttg atggtggtgg ccatctgtct
     1501 tgctctttcg taacaactta caggtcaaaa aagaccgtcg ggaacatcaa gatgcccggt
     1561 atccatgccg ttgatcaccg cctggaaagg ttagaggaaa gtgacaatga aatgttcgta
     1621 gtacaacgcg aagtcgcagt tgccaagttc gccgggcttg gtggtgggat ggacgagctg
     1681 tacaagtaa
//
