LOCUS       sequence-91509-e         531 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bidirectional construct in the pcDNA5/FRT backbone with GFP and 
            mCherry driven by the same CMV promoter along with a Gateway cloning
            site downstream mCherry.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 531)
  AUTHORS   Oikonomou P, Goodarzi H, Tavazoie S
  TITLE     Systematic Identification of Regulatory Elements in Conserved 3' 
            UTRs of Human Transcripts.
  JOURNAL   Cell Rep. 2014 Apr 10;7(1):281-92. doi: 
            10.1016/j.celrep.2014.03.001. Epub 2014 Mar 20.
  PUBMED    24656821
REFERENCE   2  (bases 1 to 531)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2014.03.001"; journalName: "Cell Rep"; date: 
            "2014-04-10- 10"; volume: "7"; issue: "1"; pages: "281-92"
COMMENT     Sequence Label: pcDNA5-FRT-GFP-mCherry-3pGW
FEATURES             Location/Qualifiers
     source          1..531
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      116..125
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(267..285)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
ORIGIN
        1 tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc tccatagaag
       61 acaccgggac cgatccagcc tccggactct agcgtttaaa cttaagctcg cccttgccac
      121 catggtgagc aagggcgagg aggataacat ggccatcatc aaggagttca tgcgcttcaa
      181 ggtgcacatg gagggctccg tgaacggcca cgagttcgag atcgagggcg agggcgaggg
      241 ccgcccctac gagggcaccc agaccgccaa gctgaaggtg accaagggtg gccccctgcc
      301 cttcgcctgg gacatcctgt cccctcagtt catgtacggc tccaaggcct acgtgaagca
      361 ccccgccgac atccccgact acttgaagct gtccttcccc gagggcttca agtgggagcg
      421 cgtgatgaac ttcgaggacg gcggcgtggt gaccgtgacc caggactcct ccctgcagga
      481 cggcgagttc atctacaagg tgaagctgcg cggcaccaac ttcccctccg a
//
