LOCUS       sequence-91510-e         785 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bidirectional construct in the pcDNA5/FRT backbone with GFP and 
            mCherry driven by the same CMV promoter along with a Gateway cloning
            site downstream mCherry.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 785)
  AUTHORS   Oikonomou P, Goodarzi H, Tavazoie S
  TITLE     Systematic Identification of Regulatory Elements in Conserved 3' 
            UTRs of Human Transcripts.
  JOURNAL   Cell Rep. 2014 Apr 10;7(1):281-92. doi: 
            10.1016/j.celrep.2014.03.001. Epub 2014 Mar 20.
  PUBMED    24656821
REFERENCE   2  (bases 1 to 785)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2014.03.001"; journalName: "Cell Rep"; date: 
            "2014-04-10- 10"; volume: "7"; issue: "1"; pages: "281-92"
COMMENT     Sequence Label: pcDNA5-FRT-GFP-mCherry-3pGW
FEATURES             Location/Qualifiers
     source          1..785
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(120..244)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(401..418)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    407..631
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(764..783)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 cggggaagaa gtggctgatc tcagccaccg cgaaaatgac atcaaaaacg ccattaacct
       61 gatgttctgg ggaatataaa tgtcaggctc ccttatacac agccagtctg caggtcgacc
      121 atagtgactg gatatgttgt gttttacagt attatgtagt ctgtttttta tgcaaaatct
      181 aatttaatat attgatattt atatcatttt acgtttctcg ttcagctttc ttgtacaaag
      241 tggtttaatt cgaaaagctt gtcgacgata tctccagagg atcaagggcg agcttggtac
      301 cgagctcgga tccactagtc cagtgtggtg gaattctgca gatatccagc acagtggcgg
      361 ccgctcgagt ctagagggcc cgtttaaacc cgctgatcag cctcgactgt gccttctagt
      421 tgccagccat ctgttgtttg cccctccccc gtgccttcct tgaccctgga aggtgccact
      481 cccactgtcc tttcctaata aaatgaggaa attgcatcgc attgtctgag taggtgtcat
      541 tctattctgg ggggtggggt ggggcaggac agcaaggggg aggattggga agacaatagc
      601 aggcatgctg gggatgcggt gggctctatg gcttctgagg cggaaagaac cagctggggc
      661 tctagggggt atccccacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt
      721 acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc
      781 ccttc
//
