LOCUS       sequence-91515-e         841 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bidirectional construct in the pcDNA5/FRT backbone with GFP and 
            mCherry driven by the same CMV promoter along with a Gateway cloning
            site downstream mCherry.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 841)
  AUTHORS   Oikonomou P, Goodarzi H, Tavazoie S
  TITLE     Systematic Identification of Regulatory Elements in Conserved 3' 
            UTRs of Human Transcripts.
  JOURNAL   Cell Rep. 2014 Apr 10;7(1):281-92. doi: 
            10.1016/j.celrep.2014.03.001. Epub 2014 Mar 20.
  PUBMED    24656821
REFERENCE   2  (bases 1 to 841)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2014.03.001"; journalName: "Cell Rep"; date: 
            "2014-04-10- 10"; volume: "7"; issue: "1"; pages: "281-92"
COMMENT     Sequence Label: pcDNA5-FRT-GFP-mCherry-3pGW
FEATURES             Location/Qualifiers
     source          1..841
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(46..765)
                     /codon_start=1
                     /product="Aequorea coerulescens GFP"
                     /label=AcGFP1
                     /note="mammalian codon-optimized"
                     /translation="MVSKGAELFTGIVPILIELNGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLSYGVQCFSRYPDHMKQHDFFKSAMPEGYIQERTIFFEDD
                     GNYKSRAEVKFEGDTLVNRIELTGTDFKEDGNILGNKMEYNYNAHNVYIMTDKAKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMIYF
                     GFVTAAAITHGMDELYK"
     regulatory      762..771
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(785..809)
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
ORIGIN
        1 gctgattatg atcctctaga ctgcagcctc aggagatctg ggccctcact tgtacagctc
       61 atccatgccg tgggtgatgg cggcggcggt cacgaagccg aagtagatca tgtgatcgcg
      121 cttctcgttg gggtccttgg acagggcgct ctgggtggac aggtagtggt tatcgggcag
      181 cagcacaggg ccatcgccga tgggggtatt ctgctggtag tggtcggcca gctgcacgct
      241 gccatcctcg atgttgtggc ggatcttgaa gttcaccttg atgccattct tggccttgtc
      301 ggtcatgatg tacacattgt gggcgttgta gttgtactcc atcttattgc ccaggatgtt
      361 gccatcctcc ttgaaatcgg tgccggtcag ctcgatgcga ttcaccaggg tatcgccctc
      421 gaacttcacc tcggcgcgcg acttgtagtt gccgtcatcc tcgaagaaga tggtgcgctc
      481 ctggatgtag ccctcaggca tggcgctctt gaagaagtcg tgctgcttca tgtgatcggg
      541 gtagcgtgag aagcactgca cgccgtagct cagggtggtc accagggtgg gccagggcac
      601 aggcagcttg ccggtggtgc agatgaactt cagggtcagc ttgccgtagg tggcatcgcc
      661 ctcgccctcg ccgctcacgc tgaacttgtg gccattcaca tcgccattca gctcgatcag
      721 gatgggcacg atgccggtga acagctcggc gcccttgctc accatggtgg cgaattctcc
      781 aggcgatctg acggttcact aaacgagctc tgcttatata ggcctcccac cgtacacgcc
      841 a
//
