LOCUS       sequence-91521-e         656 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 656)
  TITLE     Trono Lab Constitutive Lentiviral Plasmids
REFERENCE   2  (bases 1 to 656)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pWPT-GFP
FEATURES             Location/Qualifiers
     source          1..656
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        278..296
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     278..295
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     LTR             321..501
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     protein_bind    521..554
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 cacctgtggc gccggtgatg ccggccacga tgcgtccggc gtagaggatc tggctagcga
       61 tgaccctgct gattggttcg ctgaccattt ccgggtgcgg gacggcgtta ccagaaactc
      121 agaaggttcg tccaaccaaa ccgactctga cggcagttta cgagagagat gatagggtct
      181 gcttcagtaa gccagatgct acacaattag gcttgtacat attgtcgtta gaacgcggct
      241 acaattaata cataacctta tgtatcatac acatacgatt taggtgacac tatagaatac
      301 acggaattaa ttctagatgc tgctagagat tttccacact gactaaaagg gtctgaggga
      361 tctctagtta ccagagtcac acaacagacg ggcacacact acttgaagca ctcaaggcaa
      421 gctttattga ggcttaagca gtgggttccc tagttagcca gagagctccc aggctcagat
      481 ctggtctaac cagagagacc cagtacaagc aaaaagcagc ataacttcgt ataatgtatg
      541 ctatacgaag ttattcccat ggtgaattga tcccatcttg tcttcgttgg gagtgaatta
      601 gcccttccag tccccccttt tcttttaaaa agtggctaag atctacagct gccttg
//
