LOCUS       sequence-91523-e         746 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 746)
  TITLE     Trono Lab Constitutive Lentiviral Plasmids
REFERENCE   2  (bases 1 to 746)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pWPT-GFP
FEATURES             Location/Qualifiers
     source          1..746
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     51..67
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     52..68
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(189..210)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     538..557
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 gtcaatcttt cacaaatttt gtaatccaga ggttgattat cggaattccc tcgaggtcga
       61 cggtatcgcg ataagcttga tatcgaattc gaagcttgag ctcgactagc tagctagcta
      121 ctagctagtc gagatctgag tccggacttg tacagctcgt ccatgccgag agtgatcccg
      181 gcggcggtca cgaactccag caggaccatg tgatcgcgct tctcgttggg gtctttgctc
      241 agggcggact gggtgctcag gtagtggttg tcgggcagca gcacggggcc gtcgccgatg
      301 ggggtgttct gctggtagtg gtcggcgagc tgcacgctgc cgtcctcgat gttgtggcgg
      361 atcttgaagt tcaccttgat gccgttcttc tgcttgtcgg ccatgatata gacgttgtgg
      421 ctgttgtagt tgtactccag cttgtgcccc aggatgttgc cgtcctcctt gaagtcgatg
      481 cccttcagct cgatgcggtt caccagggtg tcgccctcga acttcacctc ggcgcgggtc
      541 ttgtagttgc cgtcgtcctt gaagaagatg gtgcgctcct ggacgtagcc ttcgggcatg
      601 gcggacttga agaagtcgtg ctgcttcatg tggtcggggt agcggctgaa gcactgcacg
      661 ccgtaggtca gggtggtcac gagggtgggc cagggcacgg gcagcttgcc ggtggtgcag
      721 atgaacttca gggtcagctt gccgta
//
