LOCUS       sequence-91550-e         997 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses Arabidopsis thaliana NRT1.1 in Saccharomyces cells as a 
            C-terminal GFP his tagged protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 997)
  AUTHORS   Parker JL, Newstead S
  TITLE     Molecular basis of nitrate uptake by the plant nitrate transporter 
            NRT1.1.
  JOURNAL   Nature. 2014 Mar 6;507(7490):68-72. doi: 10.1038/nature13116. Epub 
            2014 Feb 26.
  PUBMED    24572366
REFERENCE   2  (bases 1 to 997)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nature13116"; journalName: "Nature"; date: "2014-03-6- 6"; 
            volume: "507"; issue: "7490"; pages: "68-72"
COMMENT     Sequence Label: pDDGFP2_leu2d_NRT1.1
FEATURES             Location/Qualifiers
     source          1..997
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     1..17
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1..17
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
ORIGIN
        1 tctagaacta gtggatcccc catgtctctt cctgaaacta aatctgatga tatccttctt
       61 gatgcttggg acttccaagg ccgtcccgcc gatcgctcaa aaaccggcgg ctgggccagc
      121 gccgccatga ttctttgtat tgaggccgtg gagaggctga cgacgttagg tatcggagtt
      181 aatctggtga cgtatttgac gggaactatg catttaggca atgcaactgc ggctaacacc
      241 gttaccaatt tcctcggaac ttctttcatg ctctgtctcc tcggtggctt catcgccgat
      301 acctttctcg gcaggtacct aacgattgct atattcgccg caatccaagc cacgggtgtt
      361 tcaatcttaa ctctatcaac aatcataccg ggacttcgac caccaagatg caatccaaca
      421 acgtcgtctc actgcgaaca agcaagtgga atacaactga cggtcctata cttagcctta
      481 tacctcaccg ctctaggaac gggaggcgtg aaggctagtg tctcgggttt cgggtcggac
      541 caattcgatg agaccgaacc aaaagaacga tcgaaaatga catatttctt caaccgtttc
      601 ttcttttgta tcaacgttgg ctctctttta gctgtgacgg tccttgtcta cgtacaagac
      661 gatgttggac gcaaatgggg ctatggaatt tgcgcgtttg cgatcgtgct tgcactcagc
      721 gttttcttgg ccggaacaaa ccgctaccgt ttcaagaagt tgatcggtag cccgatgacg
      781 caggttgctg cggttatcgt ggcggcgtgg aggaatagga agctcgagct gccggcagat
      841 ccgtcctatc tctacgatgt ggatgatatt attgcggcgg aaggttcgat gaagggtaaa
      901 caaaagctgc cacacactga acaattccgt tcattagata aggcagcaat aagggatcag
      961 gaagcgggag ttacctcgaa tgtattcaac aagtgga
//
