LOCUS       sequence-91556-e        1050 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses S586A VLCAD mutant in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1050)
  AUTHORS   Kabuyama Y, Suzuki T, Nakazawa N, Yamaki J, Homma MK, Homma Y
  TITLE     Dysregulation of very long chain acyl-CoA dehydrogenase coupled with
            lipid peroxidation.
  JOURNAL   Am J Physiol Cell Physiol. 2010 Jan;298(1):C107-13. doi: 
            10.1152/ajpcell.00231.2009. Epub 2009 Nov 4.
  PUBMED    19889959
REFERENCE   2  (bases 1 to 1050)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1152/ajpcell.00231.2009"; journalName: "Am J Physiol Cell 
            Physiol"; date: "2010-01"; volume: "298"; issue: "1"; pages: 
            "C107-13"
COMMENT     Sequence Label: pcDNA3-S586A-VLCAD-FLAG
FEATURES             Location/Qualifiers
     source          1..1050
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     54..73
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        54..72
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 agcagagctc tctggctaac tagagaaccc actgcttact ggcttatcga aattaatacg
       61 actcactata gggagaccca agcttggtac cgagctcgga tccactagta acggccgcca
      121 gtgtgctgga attcatgcag gcggctcgga tggccgcgag cttggggcgg cagctgctga
      181 ggctcggggg cggaagctcg cggctcacgg cgctcctggg gcagccccgg cccggccctg
      241 cccggcggcc ctatgccggg ggtgccgctc agctggctct ggacaagtca gattcccacc
      301 cctctgacgc tctgaccagg aaaaaaccgg ccaaggcgga atctaagtcc tttgctgtgg
      361 gaatgttcaa aggccagctc accacagatc aggtgttccc atacccgtcc gtgctcaacg
      421 aagagcagac acagtttctt aaagagctgg tggagcctgt gtcccgtttc ttcgaggaag
      481 tgaacgatcc cgccaagaat gacgctctgg agatggtgga ggagaccact tggcagggcc
      541 tcaaggagct gggggccttt ggtctgcaag tgcccagtga gctgggtggt gtgggccttt
      601 gcaacaccca gtacgcccgt ttggtggaga tcgtgggcat gcatgacctt ggcgtgggca
      661 ttaccctggg ggcccatcag agcatcggtt tcaaaggcat cctgctcttt ggcacaaagg
      721 cccagaaaga aaaatacctc cccaagctgg catctgggga gactgtggcc gctttctgtc
      781 taaccgagcc ctcaagcggg tcagatgcag cctccatccg aacctctgct gtgcccagcc
      841 cctgtggaaa atactatacc ctcaatggaa gcaagctttg gatcagtaat gggggcctag
      901 cagacatctt cacggtcttt gccaagacac cagttacaga tccagccaca ggagccgtga
      961 aggagaagat cacagctttt gtggtggaga ggggcttcgg gggcattacc catgggcccc
     1021 ctgagaagaa gatgggcatc aaggcttcaa
//
