LOCUS       sequence-91557-e        1077 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses S586A VLCAD mutant in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1077)
  AUTHORS   Kabuyama Y, Suzuki T, Nakazawa N, Yamaki J, Homma MK, Homma Y
  TITLE     Dysregulation of very long chain acyl-CoA dehydrogenase coupled with
            lipid peroxidation.
  JOURNAL   Am J Physiol Cell Physiol. 2010 Jan;298(1):C107-13. doi: 
            10.1152/ajpcell.00231.2009. Epub 2009 Nov 4.
  PUBMED    19889959
REFERENCE   2  (bases 1 to 1077)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1152/ajpcell.00231.2009"; journalName: "Am J Physiol Cell 
            Physiol"; date: "2010-01"; volume: "298"; issue: "1"; pages: 
            "C107-13"
COMMENT     Sequence Label: pcDNA3-S586A-VLCAD-FLAG
FEATURES             Location/Qualifiers
     source          1..1077
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(56..79)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     regulatory      79..88
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 ctatagaata gggccctcta gatgcatgct cgactctaga ggatccgccc gggctcttat
       61 cgtcgtcatc cttgtaatcc atggtggcgg ccgctctgaa gccaagtggg ttgctggtga
      121 ccacaccacc ccgctccacc aaggccttgg agatgctttt gaagttgcgg tagagctctt
      181 gctgccaggg gtcagactgc agggcggcca tgccctctcg gatccgagct gcagcctcga
      241 tacaccaggt gtcacagagc attttctcat gctgggccgt ggggtggccc tcactcaggg
      301 atcttgcggc cctcgagaga accaccacca tggcatagag gtcgatggcc ccgtctgcca
      361 gccgctgcag cagaaactgt tcattgacaa tccccttctt gtgttttatc agcttggcct
      421 ccaccacagt ggcaaactgc tccagagccc gtactgccag ctcgccactc cgactcaact
      481 ccgggtggac aagtccgctg agactcaggc cgctgcccag ccctgcccgc cgcctcagct
      541 gtttgcctgc ctctcctagc aggaggccag cattcccaaa gggattcttt agagcactgc
      601 caagcccaga gagctccttt cctttgtcca tacagccctg cagagccaca aacagccgaa
      661 gaatgtcatt tgtcccctca aagatccgga agatgcgaag atctcggagc acacgctcta
      721 ctccaggttc cttcatgaag cccatacccc ccatgatttg gatgcattca tctgtcacct
      781 tccaggctgc ctccgagcca aagattttgc tgatggcggc ctctatctgg aagtccgtgg
      841 ctccctggtc catgttagca ctcaccatgt aagccatgga ctcagttaca tactgcagca
      901 taaccatccg tgccagcttc tcctggatca gcccaaagtt gtgaattttc tccccaaact
      961 gggtacgatt agtggcatga tctaccgcct tagcaatgat gcctctcatg gtacctgcca
     1021 gggccgcagc catgccaaac cttccattgt tgaggatgtg catggcaacc ttgaagc
//
