LOCUS       sequence-91577-e         800 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Negative control shRNA vector containing non-hairpin insert..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 800)
  AUTHORS   Moffat J, Grueneberg DA, Yang X, Kim SY, Kloepfer AM, Hinkle G, 
            Piqani B, Eisenhaure TM, Luo B, Grenier JK, Carpenter AE, Foo SY, 
            Stewart SA, Stockwell BR, Hacohen N, Hahn WC, Lander ES, Sabatini 
            DM, Root DE
  TITLE     A lentiviral RNAi library for human and mouse genes applied to an 
            arrayed viral high-content screen.
  JOURNAL   Cell. 2006 Mar 24. 124(6):1283-98.
  PUBMED    16564017
REFERENCE   2  (bases 1 to 800)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2006 
            Mar 24. 124(6):1283-98."
COMMENT     Sequence Label: pLKO.1 - TRC control
FEATURES             Location/Qualifiers
     source          1..800
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     LTR             66..299
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(510..530)
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
ORIGIN
        1 actctctgat attcatttct ttgcaagtta taaatactga ataataagat gacatgaact
       61 actactgcta gagattttcc acactgacta aaagggtctg agggatctct agttaccaga
      121 gtcacacaac agacgggcac acactacttg aagcactcaa ggcaagcttt attgaggctt
      181 aagcagtggg ttccctagtt agccagagag ctcccaggct cagatctggt ctaaccagag
      241 agacccagta caagcaaaaa gcagatcttg tcttcgttgg gagtgaatta gcccttccag
      301 tccccccttt tcttttaaaa agtggctaag atctacagct gccttgtaag tcattggtct
      361 taaaggtacc gatgcatggg gtcgtgcgct cctttcggtc gggcgctgcg ggtcgtgggg
      421 cgggcgtcag gcaccgggct tgcgggtcat gcaccaggtg cgcggtcctt cgggcacctc
      481 gacgtcggcg gtgacggtga agccgagccg ctcgtagaag gggaggttgc ggggcgcgga
      541 ggtctccagg aaggcgggca ccccggcgcg ctcggccgcc tccactccgg ggagcacgac
      601 ggcgctgccc agacccttgc cctggtggtc gggcgagacg ccgacggtgg ccaggaacca
      661 cgcgggctcc ttgggccggt gcggcgccag gaggccttcc atctgttgct gcgcggccag
      721 ccgggaaccg ctcaactcgg ccatgcgcgg gccgatctcg gcgaacaccg cccccgcttc
      781 gacgctctcc ggcgtggtcc
//
