LOCUS       sequence-91599-e        9812 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  FAK fret sensor without helix.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9812)
  AUTHORS   Ritt M, Guan JL, Sivaramakrishnan S
  TITLE     Visualizing and manipulating focal adhesion kinase regulation in 
            live cells.
  JOURNAL   J Biol Chem. 2013 Mar 29;288(13):8875-86. doi: 
            10.1074/jbc.M112.421164. Epub 2013 Feb 7.
  PUBMED    23393139
REFERENCE   2  (bases 1 to 9812)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.M112.421164"; journalName: "J Biol Chem"; date: 
            "2013-03-29- 29"; volume: "288"; issue: "13"; pages: "8875-86"
COMMENT     Sequence Label: pCDNA-FRT-FAK0
FEATURES             Location/Qualifiers
     source          1..9812
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     863..882
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        863..881
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             920..943
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             join(962..964,965..1675)
                     /codon_start=1
                     /product="enhanced variant of YFP (Heikal et al., 2001)"
                     /label=Citrine
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTFGYGLMCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     CDS             join(962..964,965..1675)
                     /codon_start=1
                     /product="enhanced YFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEYFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTFGYGLMCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     CDS             2987..3007
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             join(3026..3028,3029..3739)
                     /codon_start=1
                     /product="enhanced monomeric variant of CFP (Rizzo et al., 
                     2004)"
                     /label=mCerulean
                     /note=" mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNAISDNVYITADKQKNGIKA
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(5764..5781)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5770..5994
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(6127..6146)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     protein_bind    6278..6325
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     CDS             6333..7355
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     CDS             6333..7355
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     polyA_signal    7485..7606
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7522..7541)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7576..7595
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7655..7671)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7655..7671)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7668..7690)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7679..7695
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7703..7709,7710..7727,7728..7733))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7748..7769
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7886..7903)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8057..8645)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8137..8156)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8816..9607,9608..9676))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9439..9458
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9677..9781)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc
      901 gtttaaactt aagcttatgg actacaagga cgatgacgac aagggaagcg gaggaagcgg
      961 agtgagcaag ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg agctggacgg
     1021 cgacgtaaac ggccacaagt tcagcgtgtc cggcgagggc gagggcgatg ccacctacgg
     1081 caagctgacc ctgaagttca tctgcaccac cggcaagctg cccgtgccct ggcccaccct
     1141 cgtgaccacc ttcggctacg gcctgatgtg cttcgcccgc taccccgacc acatgaagca
     1201 gcacgacttc ttcaagtccg ccatgcccga aggctacgtc caggagcgca ccatcttctt
     1261 caaggacgac ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg acaccctggt
     1321 gaaccgcatc gagctgaagg gcatcgactt caaggaggac ggcaacatcc tggggcacaa
     1381 gctggagtac aactacaaca gccacaacgt ctatatcatg gccgacaagc agaagaacgg
     1441 catcaaggtg aacttcaaga tccgccacaa catcgaggac ggcagcgtgc agctcgccga
     1501 ccactaccag cagaacaccc ccatcggcga cggccccgtg ctgctgcccg acaaccacta
     1561 cctgagctac cagtccaaac tgagcaaaga ccccaacgag aagcgcgatc acatggtcct
     1621 gctggagttc gtgaccgccg ccgggatcac tctcggcatg gacgagctgt acaagtctag
     1681 aggaagcgga ggaagcggag gaagcggagg aagcggagga agcggaatgg cagcagctta
     1741 ccttgatcca aacttgaatc atacaccaag ttcaagtgca aagacgcacc tcggtactgg
     1801 gatggagcgt tccccggggg ccatggagcg agtcctaaag gtttttcact actttgaaaa
     1861 cagcagcgag ccaacgacgt gggccagcat tatccggcat ggagatgcta ctgatgttcg
     1921 aggcataata cagaagattg tggactgtca caaagtgaaa aatgtggcct gctatgggtt
     1981 gcgactcagt catctgcagt ctgaggaggt tcactggctg cacctggaca tgggggtatc
     2041 caatgtgaga gagaaatttg aactagcaca tcctccagaa gaatggaaat atgaactgag
     2101 aattcggtac ctgcccaaag gatttctaaa ccagttcact gaggacaaac caactttaaa
     2161 ttttttctat cagcaggtga aaaatgacta tatgttagaa atagcagatc aagtggacca
     2221 ggaaattgct ttgaaactag gttgccttga aatcaggaga tcctacggag agatgagagg
     2281 caatgcatta gagaagaaat ccaactatga agtgctagaa aaagatgtcg gtttaagacg
     2341 attttttccg aagagtttgc tagattcagt gaaggccaaa acactacgaa aattaatcca
     2401 acagacattt cgacaatttg ccaacctcaa cagagaagaa agtattttga aattctttga
     2461 gatcctctct ccagtgtaca gatttgacaa ggaatgcttc aagtgtgccc ttggttcaag
     2521 ctggattatt tcagtggagc tggcaattgg cccagaggaa ggaatcagct accttacaga
     2581 caagggtgca aatccaactc acctggcaga ttttaatcaa gtacaaacta ttcagtattc
     2641 aaacagtgaa gacaaggaca gaaaagggat gttgcaactg aagatagctg gtgcacctga
     2701 gcctctgaca gtgacagcac catccttaac cattgcagag aatatggctg acttgataga
     2761 cggatactgc cgactggtga atggagccac gcaatctttt attatcaggc cacagaaaga
     2821 aggtgaaaga gctttaccat caataccaaa gctggccaac aatgagaagc aaggagtaag
     2881 gtcgcacaca gtctctgtat cagaaacaga tgactatgca gagataatag atgaagaaga
     2941 tacttataca atgggaagcg gaggaagcgg agaattcgga agcggagaaa acctgtattt
     3001 tcagggcgga agcggaggcg cgcccgtgag caagggcgag gagctgttca ccggggtggt
     3061 gcccatcctg gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga
     3121 gggcgagggc gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa
     3181 gctgcccgtg ccctggccca ccctcgtgac caccctgacc tggggcgtgc agtgcttcgc
     3241 ccgctacccc gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta
     3301 cgtccaggag cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt
     3361 gaagttcgag ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga
     3421 ggacggcaac atcctggggc acaagctgga gtacaacgcc atcagcgaca acgtctatat
     3481 caccgccgac aagcagaaga acggcatcaa ggccaacttc aagatccgcc acaacatcga
     3541 ggacggcagc gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc
     3601 cgtgctgctg cccgacaacc actacctgag cacccagtcc aagctgagca aagaccccaa
     3661 cgagaagcgc gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg
     3721 catggacgag ctgtacaagt taattaaggg aagcggagga agcggaggaa gcggaggaag
     3781 cggaccatca accagagatt atgaaattca aagggagaga attgaactgg ggcgctgcat
     3841 tggtgaagga cagtttggag atgtgcacca aggaatttac atgagtccgg aaaatccagc
     3901 tatggctgta gcaatcaaaa catgtaaaaa ctgcacctca gacagcgtta gagaaaagtt
     3961 cctacaagaa gccttaacaa tgcgtcagtt tgatcatcct cacattgtga agctcattgg
     4021 agttattaca gaaaacccag tgtggataat catggagctc tgtacacttg gagagttgag
     4081 atcgtttctg caagtaagaa aattcagctt ggacctggcc tccctcatcc tctacgctta
     4141 ccagcttagc acagcacttg cttacctaga gagcaaaaga tttgtacata gagatattgc
     4201 tgctaggaac gtgctggtat ctgccactga ctgtgtgaaa ttgggtgact ttggcttatc
     4261 ccgatacatg gaagacagta cttactataa ggcgtccaaa ggaaagttac ctatcaaatg
     4321 gatggctcca gagtcaatca acttccgacg gtttacctca gcaagcgatg tgtggatgtt
     4381 tggtgtgtgt atgtgggaga tcctgatgca tggggtaaag cccttccagg gagtgaaaaa
     4441 taatgatgtt attggtcgga ttgagaacgg tgagcggctc cccatgcctc cgaactgccc
     4501 tcccaccctc tacagcctta tgaccaagtg ctgggcatac gaccctagta gacgacccag
     4561 gtttactgaa cttaaagcac aactcagtac aatactggag gaggagaagc tgcagcaaga
     4621 ggaacgaatg agaatggaat ccaggcgaca agtcacagta tcctgggact caggaggatc
     4681 agatgaagct cctcccaagc ccagcaggcc tggttacccc agcccaaggt ccagtgaagg
     4741 gttttatccg agtcctcagc atatggtaca gccaaatcac taccaggtat ctggctactc
     4801 tggttctcat gggataccag ccatggcagg cagcatttat cctgggcagg cgtctctctt
     4861 ggatcaaaca gattcctgga accatcgacc tcaggaagta tcagcatggc agccaaacat
     4921 ggaggattcg ggcactttgg atgtacgagg aatggggcag gttctgccca cacatctcat
     4981 ggaggagagg ttaataagac aacagcaaga gatggaagaa gatcaacgct ggcttgagaa
     5041 agaggaacga ttcctggtaa tgaaacctga tgtgcggctc tccagaggca gcattgaacg
     5101 ggaggacgga ggtctccagg gcccagctgg taaccagcac atatatcagc ctgtgggtaa
     5161 accagatcat gccgctccac caaagaagcc ccctcgccct ggagcccccc acttgggcag
     5221 cctcgcgagc ctgaacagcc ccgtggacag ctacaacgaa ggcgtgaaga tcaagccaca
     5281 ggaaatcagc cctcctccta cggccaacct ggaccgctcc aatgacaaag tctatgagaa
     5341 tgtaaccggg ctggtgaaag ctgtcataga gatgtccagt aaaatacagc cagctccgcc
     5401 agaggagtac gtgcccatgg taaaggaggt tggcttggcg ctgagaacct tgctagcaac
     5461 agtggatgag tcgctgccag tgcttcctgc aagcacccac agagagattg agatggccca
     5521 gaaactgctg aactctgacc tggctgagct cattaacaag atgaagctgg cccagcagta
     5581 cgtcatgacc agcctgcagc aggagtacaa gaagcaaatg ctgacggctg ctcacgctct
     5641 ggctgtggat gccaagaact tgctggatgt catcgatcaa gccagactga aaatgatcag
     5701 ccagtccagg ccccactgag cggccgctcg agtctagagg gcccgtttaa acccgctgat
     5761 cagcctcgac tgtgccttct agttgccagc catctgttgt ttgcccctcc cccgtgcctt
     5821 ccttgaccct ggaaggtgcc actcccactg tcctttccta ataaaatgag gaaattgcat
     5881 cgcattgtct gagtaggtgt cattctattc tggggggtgg ggtggggcag gacagcaagg
     5941 gggaggattg ggaagacaat agcaggcatg ctggggatgc ggtgggctct atggcttctg
     6001 aggcggaaag aaccagctgg ggctctaggg ggtatcccca cgcgccctgt agcggcgcat
     6061 taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccctag
     6121 cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc
     6181 aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg cacctcgacc
     6241 ccaaaaaact tgattagggt gatggttcac gtacctagaa gttcctattc cgaagttcct
     6301 attctctaga aagtatagga acttccttgg ccaaaaagcc tgaactcacc gcgacgtctg
     6361 tcgagaagtt tctgatcgaa aagttcgaca gcgtctccga cctgatgcag ctctcggagg
     6421 gcgaagaatc tcgtgctttc agcttcgatg taggagggcg tggatatgtc ctgcgggtaa
     6481 atagctgcgc cgatggtttc tacaaagatc gttatgttta tcggcacttt gcatcggccg
     6541 cgctcccgat tccggaagtg cttgacattg gggaattcag cgagagcctg acctattgca
     6601 tctcccgccg tgcacagggt gtcacgttgc aagacctgcc tgaaaccgaa ctgcccgctg
     6661 ttctgcagcc ggtcgcggag gccatggatg cgatcgctgc ggccgatctt agccagacga
     6721 gcgggttcgg cccattcgga ccgcaaggaa tcggtcaata cactacatgg cgtgatttca
     6781 tatgcgcgat tgctgatccc catgtgtatc actggcaaac tgtgatggac gacaccgtca
     6841 gtgcgtccgt cgcgcaggct ctcgatgagc tgatgctttg ggccgaggac tgccccgaag
     6901 tccggcacct cgtgcacgcg gatttcggct ccaacaatgt cctgacggac aatggccgca
     6961 taacagcggt cattgactgg agcgaggcga tgttcgggga ttcccaatac gaggtcgcca
     7021 acatcttctt ctggaggccg tggttggctt gtatggagca gcagacgcgc tacttcgagc
     7081 ggaggcatcc ggagcttgca ggatcgccgc ggctccgggc gtatatgctc cgcattggtc
     7141 ttgaccaact ctatcagagc ttggttgacg gcaatttcga tgatgcagct tgggcgcagg
     7201 gtcgatgcga cgcaatcgtc cgatccggag ccgggactgt cgggcgtaca caaatcgccc
     7261 gcagaagcgc ggccgtctgg accgatggct gtgtagaagt actcgccgat agtggaaacc
     7321 gacgccccag cactcgtccg agggcaaagg aatagcacgt actacgagat ttcgattcca
     7381 ccgccgcctt ctatgaaagg ttgggcttcg gaatcgtttt ccgggacgcc ggctggatga
     7441 tcctccagcg cggggatctc atgctggagt tcttcgccca ccccaacttg tttattgcag
     7501 cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt
     7561 cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctgtatac
     7621 cgtcgacctc tagctagagc ttggcgtaat catggtcata gctgtttcct gtgtgaaatt
     7681 gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg
     7741 gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt
     7801 cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt
     7861 tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc
     7921 tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg
     7981 ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg
     8041 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac
     8101 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg
     8161 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct
     8221 ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg
     8281 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct
     8341 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac
     8401 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt
     8461 tcttgaagtg gtggcctaac tacggctaca ctagaaggac agtatttggt atctgcgctc
     8521 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca
     8581 ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat
     8641 ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac
     8701 gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt
     8761 aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc
     8821 aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg
     8881 cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg
     8941 ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc
     9001 cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta
     9061 ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg
     9121 ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct
     9181 ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta
     9241 gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg
     9301 ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga
     9361 ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt
     9421 gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca
     9481 ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt
     9541 cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt
     9601 ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga
     9661 aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat cagggttatt
     9721 gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc
     9781 gcacatttcc ccgaaaagtg ccacctgacg tc
//
