LOCUS       sequence-91604-e       10058 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  FAK fret sensor with 10 nm helix.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10058)
  AUTHORS   Ritt M, Guan JL, Sivaramakrishnan S
  TITLE     Visualizing and manipulating focal adhesion kinase regulation in 
            live cells.
  JOURNAL   J Biol Chem. 2013 Mar 29;288(13):8875-86. doi: 
            10.1074/jbc.M112.421164. Epub 2013 Feb 7.
  PUBMED    23393139
REFERENCE   2  (bases 1 to 10058)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.M112.421164"; journalName: "J Biol Chem"; date: 
            "2013-03-29- 29"; volume: "288"; issue: "13"; pages: "8875-86"
COMMENT     Sequence Label: pCDNA-FRT-FAK10
FEATURES             Location/Qualifiers
     source          1..10058
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     863..882
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        863..881
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             920..943
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             join(962..964,965..1675)
                     /codon_start=1
                     /product="enhanced variant of YFP (Heikal et al., 2001)"
                     /label=Citrine
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTFGYGLMCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     CDS             join(962..964,965..1675)
                     /codon_start=1
                     /product="enhanced YFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEYFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTFGYGLMCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     CDS             2996..3016
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             join(3272..3274,3275..3985)
                     /codon_start=1
                     /product="enhanced monomeric variant of CFP (Rizzo et al., 
                     2004)"
                     /label=mCerulean
                     /note=" mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNAISDNVYITADKQKNGIKA
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(6010..6027)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6016..6240
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(6373..6392)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     protein_bind    6524..6571
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     CDS             6579..7601
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     CDS             6579..7601
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     polyA_signal    7731..7852
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7768..7787)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7822..7841
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7901..7917)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7901..7917)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7914..7936)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7925..7941
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7949..7955,7956..7973,7974..7979))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7994..8015
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8132..8149)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8303..8891)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8383..8402)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9062..9853,9854..9922))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9685..9704
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9923..10027)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc
      901 gtttaaactt aagcttatgg actacaagga cgatgacgac aagggaagcg gaggaagcgg
      961 agtgagcaag ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg agctggacgg
     1021 cgacgtaaac ggccacaagt tcagcgtgtc cggcgagggc gagggcgatg ccacctacgg
     1081 caagctgacc ctgaagttca tctgcaccac cggcaagctg cccgtgccct ggcccaccct
     1141 cgtgaccacc ttcggctacg gcctgatgtg cttcgcccgc taccccgacc acatgaagca
     1201 gcacgacttc ttcaagtccg ccatgcccga aggctacgtc caggagcgca ccatcttctt
     1261 caaggacgac ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg acaccctggt
     1321 gaaccgcatc gagctgaagg gcatcgactt caaggaggac ggcaacatcc tggggcacaa
     1381 gctggagtac aactacaaca gccacaacgt ctatatcatg gccgacaagc agaagaacgg
     1441 catcaaggtg aacttcaaga tccgccacaa catcgaggac ggcagcgtgc agctcgccga
     1501 ccactaccag cagaacaccc ccatcggcga cggccccgtg ctgctgcccg acaaccacta
     1561 cctgagctac cagtccaaac tgagcaaaga ccccaacgag aagcgcgatc acatggtcct
     1621 gctggagttc gtgaccgccg ccgggatcac tctcggcatg gacgagctgt acaagtctag
     1681 aggaagcgga ggaagcggag gaagcggagg aagcggagga agcggaatgg cagcagctta
     1741 ccttgatcca aacttgaatc atacaccaag ttcaagtgca aagacgcacc tcggtactgg
     1801 gatggagcgt tccccggggg ccatggagcg agtcctaaag gtttttcact actttgaaaa
     1861 cagcagcgag ccaacgacgt gggccagcat tatccggcat ggagatgcta ctgatgttcg
     1921 aggcataata cagaagattg tggactgtca caaagtgaaa aatgtggcct gctatgggtt
     1981 gcgactcagt catctgcagt ctgaggaggt tcactggctg cacctggaca tgggggtatc
     2041 caatgtgaga gagaaatttg aactagcaca tcctccagaa gaatggaaat atgaactgag
     2101 aattcggtac ctgcccaaag gatttctaaa ccagttcact gaggacaaac caactttaaa
     2161 ttttttctat cagcaggtga aaaatgacta tatgttagaa atagcagatc aagtggacca
     2221 ggaaattgct ttgaaactag gttgccttga aatcaggaga tcctacggag agatgagagg
     2281 caatgcatta gagaagaaat ccaactatga agtgctagaa aaagatgtcg gtttaagacg
     2341 attttttccg aagagtttgc tagattcagt gaaggccaaa acactacgaa aattaatcca
     2401 acagacattt cgacaatttg ccaacctcaa cagagaagaa agtattttga aattctttga
     2461 gatcctctct ccagtgtaca gatttgacaa ggaatgcttc aagtgtgccc ttggttcaag
     2521 ctggattatt tcagtggagc tggcaattgg cccagaggaa ggaatcagct accttacaga
     2581 caagggtgca aatccaactc acctggcaga ttttaatcaa gtacaaacta ttcagtattc
     2641 aaacagtgaa gacaaggaca gaaaagggat gttgcaactg aagatagctg gtgcacctga
     2701 gcctctgaca gtgacagcac catccttaac cattgcagag aatatggctg acttgataga
     2761 cggatactgc cgactggtga atggagccac gcaatctttt attatcaggc cacagaaaga
     2821 aggtgaaaga gctttaccat caataccaaa gctggccaac aatgagaagc aaggagtaag
     2881 gtcgcacaca gtctctgtat cagaaacaga tgactatgca gagataatag atgaagaaga
     2941 tacttataca atgggaagcg gaggaagcgg agaattcgga agcggaggaa gcggagaaaa
     3001 cctgtatttt cagggcggaa gcggagaaga ggaagagaaa aaaaaacagc aggaagagga
     3061 agcagaaagg ctgaggcgta ttcaagaaga aatggaaaag gaaagaaaaa gacgtgaaga
     3121 agatgaacaa cgtcgaagaa aggaggagga ggaaaggcgg atgaaacttg agatggaagc
     3181 aaagagaaaa caagaagaag aagagagaaa gaaaagggaa gatgatgaaa aacgcaagaa
     3241 gaagggctct ggcggctctg gcggcgcgcc cgtgagcaag ggcgaggagc tgttcaccgg
     3301 ggtggtgccc atcctggtcg agctggacgg cgacgtaaac ggccacaagt tcagcgtgtc
     3361 cggcgagggc gagggcgatg ccacctacgg caagctgacc ctgaagttca tctgcaccac
     3421 cggcaagctg cccgtgccct ggcccaccct cgtgaccacc ctgacctggg gcgtgcagtg
     3481 cttcgcccgc taccccgacc acatgaagca gcacgacttc ttcaagtccg ccatgcccga
     3541 aggctacgtc caggagcgca ccatcttctt caaggacgac ggcaactaca agacccgcgc
     3601 cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc gagctgaagg gcatcgactt
     3661 caaggaggac ggcaacatcc tggggcacaa gctggagtac aacgccatca gcgacaacgt
     3721 ctatatcacc gccgacaagc agaagaacgg catcaaggcc aacttcaaga tccgccacaa
     3781 catcgaggac ggcagcgtgc agctcgccga ccactaccag cagaacaccc ccatcggcga
     3841 cggccccgtg ctgctgcccg acaaccacta cctgagcacc cagtccaagc tgagcaaaga
     3901 ccccaacgag aagcgcgatc acatggtcct gctggagttc gtgaccgccg ccgggatcac
     3961 tctcggcatg gacgagctgt acaagttaat taagggaagc ggaggaagcg gaggaagcgg
     4021 aggaagcgga ccatcaacca gagattatga aattcaaagg gagagaattg aactggggcg
     4081 ctgcattggt gaaggacagt ttggagatgt gcaccaagga atttacatga gtccggaaaa
     4141 tccagctatg gctgtagcaa tcaaaacatg taaaaactgc acctcagaca gcgttagaga
     4201 aaagttccta caagaagcct taacaatgcg tcagtttgat catcctcaca ttgtgaagct
     4261 cattggagtt attacagaaa acccagtgtg gataatcatg gagctctgta cacttggaga
     4321 gttgagatcg tttctgcaag taagaaaatt cagcttggac ctggcctccc tcatcctcta
     4381 cgcttaccag cttagcacag cacttgctta cctagagagc aaaagatttg tacatagaga
     4441 tattgctgct aggaacgtgc tggtatctgc cactgactgt gtgaaattgg gtgactttgg
     4501 cttatcccga tacatggaag acagtactta ctataaggcg tccaaaggaa agttacctat
     4561 caaatggatg gctccagagt caatcaactt ccgacggttt acctcagcaa gcgatgtgtg
     4621 gatgtttggt gtgtgtatgt gggagatcct gatgcatggg gtaaagccct tccagggagt
     4681 gaaaaataat gatgttattg gtcggattga gaacggtgag cggctcccca tgcctccgaa
     4741 ctgccctccc accctctaca gccttatgac caagtgctgg gcatacgacc ctagtagacg
     4801 acccaggttt actgaactta aagcacaact cagtacaata ctggaggagg agaagctgca
     4861 gcaagaggaa cgaatgagaa tggaatccag gcgacaagtc acagtatcct gggactcagg
     4921 aggatcagat gaagctcctc ccaagcccag caggcctggt taccccagcc caaggtccag
     4981 tgaagggttt tatccgagtc ctcagcatat ggtacagcca aatcactacc aggtatctgg
     5041 ctactctggt tctcatggga taccagccat ggcaggcagc atttatcctg ggcaggcgtc
     5101 tctcttggat caaacagatt cctggaacca tcgacctcag gaagtatcag catggcagcc
     5161 aaacatggag gattcgggca ctttggatgt acgaggaatg gggcaggttc tgcccacaca
     5221 tctcatggag gagaggttaa taagacaaca gcaagagatg gaagaagatc aacgctggct
     5281 tgagaaagag gaacgattcc tggtaatgaa acctgatgtg cggctctcca gaggcagcat
     5341 tgaacgggag gacggaggtc tccagggccc agctggtaac cagcacatat atcagcctgt
     5401 gggtaaacca gatcatgccg ctccaccaaa gaagccccct cgccctggag ccccccactt
     5461 gggcagcctc gcgagcctga acagccccgt ggacagctac aacgaaggcg tgaagatcaa
     5521 gccacaggaa atcagccctc ctcctacggc caacctggac cgctccaatg acaaagtcta
     5581 tgagaatgta accgggctgg tgaaagctgt catagagatg tccagtaaaa tacagccagc
     5641 tccgccagag gagtacgtgc ccatggtaaa ggaggttggc ttggcgctga gaaccttgct
     5701 agcaacagtg gatgagtcgc tgccagtgct tcctgcaagc acccacagag agattgagat
     5761 ggcccagaaa ctgctgaact ctgacctggc tgagctcatt aacaagatga agctggccca
     5821 gcagtacgtc atgaccagcc tgcagcagga gtacaagaag caaatgctga cggctgctca
     5881 cgctctggct gtggatgcca agaacttgct ggatgtcatc gatcaagcca gactgaaaat
     5941 gatcagccag tccaggcccc actgagcggc cgctcgagtc tagagggccc gtttaaaccc
     6001 gctgatcagc ctcgactgtg ccttctagtt gccagccatc tgttgtttgc ccctcccccg
     6061 tgccttcctt gaccctggaa ggtgccactc ccactgtcct ttcctaataa aatgaggaaa
     6121 ttgcatcgca ttgtctgagt aggtgtcatt ctattctggg gggtggggtg gggcaggaca
     6181 gcaaggggga ggattgggaa gacaatagca ggcatgctgg ggatgcggtg ggctctatgg
     6241 cttctgaggc ggaaagaacc agctggggct ctagggggta tccccacgcg ccctgtagcg
     6301 gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg
     6361 ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc
     6421 cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct ttacggcacc
     6481 tcgaccccaa aaaacttgat tagggtgatg gttcacgtac ctagaagttc ctattccgaa
     6541 gttcctattc tctagaaagt ataggaactt ccttggccaa aaagcctgaa ctcaccgcga
     6601 cgtctgtcga gaagtttctg atcgaaaagt tcgacagcgt ctccgacctg atgcagctct
     6661 cggagggcga agaatctcgt gctttcagct tcgatgtagg agggcgtgga tatgtcctgc
     6721 gggtaaatag ctgcgccgat ggtttctaca aagatcgtta tgtttatcgg cactttgcat
     6781 cggccgcgct cccgattccg gaagtgcttg acattgggga attcagcgag agcctgacct
     6841 attgcatctc ccgccgtgca cagggtgtca cgttgcaaga cctgcctgaa accgaactgc
     6901 ccgctgttct gcagccggtc gcggaggcca tggatgcgat cgctgcggcc gatcttagcc
     6961 agacgagcgg gttcggccca ttcggaccgc aaggaatcgg tcaatacact acatggcgtg
     7021 atttcatatg cgcgattgct gatccccatg tgtatcactg gcaaactgtg atggacgaca
     7081 ccgtcagtgc gtccgtcgcg caggctctcg atgagctgat gctttgggcc gaggactgcc
     7141 ccgaagtccg gcacctcgtg cacgcggatt tcggctccaa caatgtcctg acggacaatg
     7201 gccgcataac agcggtcatt gactggagcg aggcgatgtt cggggattcc caatacgagg
     7261 tcgccaacat cttcttctgg aggccgtggt tggcttgtat ggagcagcag acgcgctact
     7321 tcgagcggag gcatccggag cttgcaggat cgccgcggct ccgggcgtat atgctccgca
     7381 ttggtcttga ccaactctat cagagcttgg ttgacggcaa tttcgatgat gcagcttggg
     7441 cgcagggtcg atgcgacgca atcgtccgat ccggagccgg gactgtcggg cgtacacaaa
     7501 tcgcccgcag aagcgcggcc gtctggaccg atggctgtgt agaagtactc gccgatagtg
     7561 gaaaccgacg ccccagcact cgtccgaggg caaaggaata gcacgtacta cgagatttcg
     7621 attccaccgc cgccttctat gaaaggttgg gcttcggaat cgttttccgg gacgccggct
     7681 ggatgatcct ccagcgcggg gatctcatgc tggagttctt cgcccacccc aacttgttta
     7741 ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat
     7801 ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct
     7861 gtataccgtc gacctctagc tagagcttgg cgtaatcatg gtcatagctg tttcctgtgt
     7921 gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag
     7981 cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt
     8041 tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag
     8101 gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg
     8161 ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat
     8221 caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta
     8281 aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa
     8341 atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc
     8401 cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt
     8461 ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca
     8521 gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg
     8581 accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat
     8641 cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta
     8701 cagagttctt gaagtggtgg cctaactacg gctacactag aaggacagta tttggtatct
     8761 gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac
     8821 aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa
     8881 aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa
     8941 actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt
     9001 taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca
     9061 gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca
     9121 tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc
     9181 ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa
     9241 accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc
     9301 agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca
     9361 acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat
     9421 tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag
     9481 cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac
     9541 tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt
     9601 ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt
     9661 gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc
     9721 tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat
     9781 ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca
     9841 gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga
     9901 cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg
     9961 gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg
    10021 ttccgcgcac atttccccga aaagtgccac ctgacgtc
//
