LOCUS       sequence-91609-e       10457 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  FAK fret sensor with 30 nm helix.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10457)
  AUTHORS   Ritt M, Guan JL, Sivaramakrishnan S
  TITLE     Visualizing and manipulating focal adhesion kinase regulation in 
            live cells.
  JOURNAL   J Biol Chem. 2013 Mar 29;288(13):8875-86. doi: 
            10.1074/jbc.M112.421164. Epub 2013 Feb 7.
  PUBMED    23393139
REFERENCE   2  (bases 1 to 10457)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.M112.421164"; journalName: "J Biol Chem"; date: 
            "2013-03-29- 29"; volume: "288"; issue: "13"; pages: "8875-86"
COMMENT     Sequence Label: pCDNA-FRT-FAK30
FEATURES             Location/Qualifiers
     source          1..10457
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     863..882
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        863..881
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             920..943
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             join(962..964,965..1675)
                     /codon_start=1
                     /product="enhanced variant of YFP (Heikal et al., 2001)"
                     /label=Citrine
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTFGYGLMCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     CDS             join(962..964,965..1675)
                     /codon_start=1
                     /product="enhanced YFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEYFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTFGYGLMCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     CDS             2996..3016
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             join(3671..3673,3674..4384)
                     /codon_start=1
                     /product="enhanced monomeric variant of CFP (Rizzo et al., 
                     2004)"
                     /label=mCerulean
                     /note=" mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNAISDNVYITADKQKNGIKA
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(6409..6426)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6415..6639
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(6772..6791)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     protein_bind    6923..6970
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     CDS             6978..8000
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     CDS             6978..8000
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     polyA_signal    8130..8251
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8167..8186)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8221..8240
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(8300..8316)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8300..8316)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8313..8335)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8324..8340
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8348..8354,8355..8372,8373..8378))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8393..8414
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8531..8548)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8702..9290)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8782..8801)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9461..10252,10253..10321))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10084..10103
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10322..10426)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc
      901 gtttaaactt aagcttatgg actacaagga cgatgacgac aagggaagcg gaggaagcgg
      961 agtgagcaag ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg agctggacgg
     1021 cgacgtaaac ggccacaagt tcagcgtgtc cggcgagggc gagggcgatg ccacctacgg
     1081 caagctgacc ctgaagttca tctgcaccac cggcaagctg cccgtgccct ggcccaccct
     1141 cgtgaccacc ttcggctacg gcctgatgtg cttcgcccgc taccccgacc acatgaagca
     1201 gcacgacttc ttcaagtccg ccatgcccga aggctacgtc caggagcgca ccatcttctt
     1261 caaggacgac ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg acaccctggt
     1321 gaaccgcatc gagctgaagg gcatcgactt caaggaggac ggcaacatcc tggggcacaa
     1381 gctggagtac aactacaaca gccacaacgt ctatatcatg gccgacaagc agaagaacgg
     1441 catcaaggtg aacttcaaga tccgccacaa catcgaggac ggcagcgtgc agctcgccga
     1501 ccactaccag cagaacaccc ccatcggcga cggccccgtg ctgctgcccg acaaccacta
     1561 cctgagctac cagtccaaac tgagcaaaga ccccaacgag aagcgcgatc acatggtcct
     1621 gctggagttc gtgaccgccg ccgggatcac tctcggcatg gacgagctgt acaagtctag
     1681 aggaagcgga ggaagcggag gaagcggagg aagcggagga agcggaatgg cagcagctta
     1741 ccttgatcca aacttgaatc atacaccaag ttcaagtgca aagacgcacc tcggtactgg
     1801 gatggagcgt tccccggggg ccatggagcg agtcctaaag gtttttcact actttgaaaa
     1861 cagcagcgag ccaacgacgt gggccagcat tatccggcat ggagatgcta ctgatgttcg
     1921 aggcataata cagaagattg tggactgtca caaagtgaaa aatgtggcct gctatgggtt
     1981 gcgactcagt catctgcagt ctgaggaggt tcactggctg cacctggaca tgggggtatc
     2041 caatgtgaga gagaaatttg aactagcaca tcctccagaa gaatggaaat atgaactgag
     2101 aattcggtac ctgcccaaag gatttctaaa ccagttcact gaggacaaac caactttaaa
     2161 ttttttctat cagcaggtga aaaatgacta tatgttagaa atagcagatc aagtggacca
     2221 ggaaattgct ttgaaactag gttgccttga aatcaggaga tcctacggag agatgagagg
     2281 caatgcatta gagaagaaat ccaactatga agtgctagaa aaagatgtcg gtttaagacg
     2341 attttttccg aagagtttgc tagattcagt gaaggccaaa acactacgaa aattaatcca
     2401 acagacattt cgacaatttg ccaacctcaa cagagaagaa agtattttga aattctttga
     2461 gatcctctct ccagtgtaca gatttgacaa ggaatgcttc aagtgtgccc ttggttcaag
     2521 ctggattatt tcagtggagc tggcaattgg cccagaggaa ggaatcagct accttacaga
     2581 caagggtgca aatccaactc acctggcaga ttttaatcaa gtacaaacta ttcagtattc
     2641 aaacagtgaa gacaaggaca gaaaagggat gttgcaactg aagatagctg gtgcacctga
     2701 gcctctgaca gtgacagcac catccttaac cattgcagag aatatggctg acttgataga
     2761 cggatactgc cgactggtga atggagccac gcaatctttt attatcaggc cacagaaaga
     2821 aggtgaaaga gctttaccat caataccaaa gctggccaac aatgagaagc aaggagtaag
     2881 gtcgcacaca gtctctgtat cagaaacaga tgactatgca gagataatag atgaagaaga
     2941 tacttataca atgggaagcg gaggaagcgg agaattcgga agcggaggaa gcggagaaaa
     3001 cctgtatttt cagggcggaa gcggagaaga ggaagagaag aagaaagaag aggaagaaaa
     3061 gaaacaaaaa gaagaacaag aaagacttgc aaaagaagag gcagagagaa aacaaaaaga
     3121 agaacaagaa agacttgcaa aagaagaggc agagagaaaa caaaaggagg aagaagagag
     3181 aaaacaaaag gaagaagaag agagaaaaca aaaggaggaa gaagaaagaa aattaaagga
     3241 ggaacaagaa agaaaagctg cagaagaaaa gaaagctaaa gaagaagctg agagaaaggc
     3301 taaagaagaa caagaaagga aagctgaaga agagagaaag aagaaagaag aggaagaaag
     3361 acttgaaaga gaaagaaaag agagagaaga acaagaaaag aaagccaaag aagaggcaga
     3421 gagaattgca aagttagagg ctgaaaagaa ggcagaagaa gaaagaaaag ccaaagaaga
     3481 agaagagaga aaagccaaag aagaagagga aagaaagaag aaagaggagc aagaaagact
     3541 tgcaaaagaa aaggaagaag cagaaagaaa agctgcagag gaaaagaaag ctaaagaaga
     3601 acaagaaaga aaagaaaagg aagaagcaga aagaaaacaa agaggctctg gcggctctgg
     3661 cggcgcgccc gtgagcaagg gcgaggagct gttcaccggg gtggtgccca tcctggtcga
     3721 gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc ggcgagggcg agggcgatgc
     3781 cacctacggc aagctgaccc tgaagttcat ctgcaccacc ggcaagctgc ccgtgccctg
     3841 gcccaccctc gtgaccaccc tgacctgggg cgtgcagtgc ttcgcccgct accccgacca
     3901 catgaagcag cacgacttct tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac
     3961 catcttcttc aaggacgacg gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga
     4021 caccctggtg aaccgcatcg agctgaaggg catcgacttc aaggaggacg gcaacatcct
     4081 ggggcacaag ctggagtaca acgccatcag cgacaacgtc tatatcaccg ccgacaagca
     4141 gaagaacggc atcaaggcca acttcaagat ccgccacaac atcgaggacg gcagcgtgca
     4201 gctcgccgac cactaccagc agaacacccc catcggcgac ggccccgtgc tgctgcccga
     4261 caaccactac ctgagcaccc agtccaagct gagcaaagac cccaacgaga agcgcgatca
     4321 catggtcctg ctggagttcg tgaccgccgc cgggatcact ctcggcatgg acgagctgta
     4381 caagttaatt aagggaagcg gaggaagcgg aggaagcgga ggaagcggac catcaaccag
     4441 agattatgaa attcaaaggg agagaattga actggggcgc tgcattggtg aaggacagtt
     4501 tggagatgtg caccaaggaa tttacatgag tccggaaaat ccagctatgg ctgtagcaat
     4561 caaaacatgt aaaaactgca cctcagacag cgttagagaa aagttcctac aagaagcctt
     4621 aacaatgcgt cagtttgatc atcctcacat tgtgaagctc attggagtta ttacagaaaa
     4681 cccagtgtgg ataatcatgg agctctgtac acttggagag ttgagatcgt ttctgcaagt
     4741 aagaaaattc agcttggacc tggcctccct catcctctac gcttaccagc ttagcacagc
     4801 acttgcttac ctagagagca aaagatttgt acatagagat attgctgcta ggaacgtgct
     4861 ggtatctgcc actgactgtg tgaaattggg tgactttggc ttatcccgat acatggaaga
     4921 cagtacttac tataaggcgt ccaaaggaaa gttacctatc aaatggatgg ctccagagtc
     4981 aatcaacttc cgacggttta cctcagcaag cgatgtgtgg atgtttggtg tgtgtatgtg
     5041 ggagatcctg atgcatgggg taaagccctt ccagggagtg aaaaataatg atgttattgg
     5101 tcggattgag aacggtgagc ggctccccat gcctccgaac tgccctccca ccctctacag
     5161 ccttatgacc aagtgctggg catacgaccc tagtagacga cccaggttta ctgaacttaa
     5221 agcacaactc agtacaatac tggaggagga gaagctgcag caagaggaac gaatgagaat
     5281 ggaatccagg cgacaagtca cagtatcctg ggactcagga ggatcagatg aagctcctcc
     5341 caagcccagc aggcctggtt accccagccc aaggtccagt gaagggtttt atccgagtcc
     5401 tcagcatatg gtacagccaa atcactacca ggtatctggc tactctggtt ctcatgggat
     5461 accagccatg gcaggcagca tttatcctgg gcaggcgtct ctcttggatc aaacagattc
     5521 ctggaaccat cgacctcagg aagtatcagc atggcagcca aacatggagg attcgggcac
     5581 tttggatgta cgaggaatgg ggcaggttct gcccacacat ctcatggagg agaggttaat
     5641 aagacaacag caagagatgg aagaagatca acgctggctt gagaaagagg aacgattcct
     5701 ggtaatgaaa cctgatgtgc ggctctccag aggcagcatt gaacgggagg acggaggtct
     5761 ccagggccca gctggtaacc agcacatata tcagcctgtg ggtaaaccag atcatgccgc
     5821 tccaccaaag aagccccctc gccctggagc cccccacttg ggcagcctcg cgagcctgaa
     5881 cagccccgtg gacagctaca acgaaggcgt gaagatcaag ccacaggaaa tcagccctcc
     5941 tcctacggcc aacctggacc gctccaatga caaagtctat gagaatgtaa ccgggctggt
     6001 gaaagctgtc atagagatgt ccagtaaaat acagccagct ccgccagagg agtacgtgcc
     6061 catggtaaag gaggttggct tggcgctgag aaccttgcta gcaacagtgg atgagtcgct
     6121 gccagtgctt cctgcaagca cccacagaga gattgagatg gcccagaaac tgctgaactc
     6181 tgacctggct gagctcatta acaagatgaa gctggcccag cagtacgtca tgaccagcct
     6241 gcagcaggag tacaagaagc aaatgctgac ggctgctcac gctctggctg tggatgccaa
     6301 gaacttgctg gatgtcatcg atcaagccag actgaaaatg atcagccagt ccaggcccca
     6361 ctgagcggcc gctcgagtct agagggcccg tttaaacccg ctgatcagcc tcgactgtgc
     6421 cttctagttg ccagccatct gttgtttgcc cctcccccgt gccttccttg accctggaag
     6481 gtgccactcc cactgtcctt tcctaataaa atgaggaaat tgcatcgcat tgtctgagta
     6541 ggtgtcattc tattctgggg ggtggggtgg ggcaggacag caagggggag gattgggaag
     6601 acaatagcag gcatgctggg gatgcggtgg gctctatggc ttctgaggcg gaaagaacca
     6661 gctggggctc tagggggtat ccccacgcgc cctgtagcgg cgcattaagc gcggcgggtg
     6721 tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc gctcctttcg
     6781 ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg
     6841 ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa aaacttgatt
     6901 agggtgatgg ttcacgtacc tagaagttcc tattccgaag ttcctattct ctagaaagta
     6961 taggaacttc cttggccaaa aagcctgaac tcaccgcgac gtctgtcgag aagtttctga
     7021 tcgaaaagtt cgacagcgtc tccgacctga tgcagctctc ggagggcgaa gaatctcgtg
     7081 ctttcagctt cgatgtagga gggcgtggat atgtcctgcg ggtaaatagc tgcgccgatg
     7141 gtttctacaa agatcgttat gtttatcggc actttgcatc ggccgcgctc ccgattccgg
     7201 aagtgcttga cattggggaa ttcagcgaga gcctgaccta ttgcatctcc cgccgtgcac
     7261 agggtgtcac gttgcaagac ctgcctgaaa ccgaactgcc cgctgttctg cagccggtcg
     7321 cggaggccat ggatgcgatc gctgcggccg atcttagcca gacgagcggg ttcggcccat
     7381 tcggaccgca aggaatcggt caatacacta catggcgtga tttcatatgc gcgattgctg
     7441 atccccatgt gtatcactgg caaactgtga tggacgacac cgtcagtgcg tccgtcgcgc
     7501 aggctctcga tgagctgatg ctttgggccg aggactgccc cgaagtccgg cacctcgtgc
     7561 acgcggattt cggctccaac aatgtcctga cggacaatgg ccgcataaca gcggtcattg
     7621 actggagcga ggcgatgttc ggggattccc aatacgaggt cgccaacatc ttcttctgga
     7681 ggccgtggtt ggcttgtatg gagcagcaga cgcgctactt cgagcggagg catccggagc
     7741 ttgcaggatc gccgcggctc cgggcgtata tgctccgcat tggtcttgac caactctatc
     7801 agagcttggt tgacggcaat ttcgatgatg cagcttgggc gcagggtcga tgcgacgcaa
     7861 tcgtccgatc cggagccggg actgtcgggc gtacacaaat cgcccgcaga agcgcggccg
     7921 tctggaccga tggctgtgta gaagtactcg ccgatagtgg aaaccgacgc cccagcactc
     7981 gtccgagggc aaaggaatag cacgtactac gagatttcga ttccaccgcc gccttctatg
     8041 aaaggttggg cttcggaatc gttttccggg acgccggctg gatgatcctc cagcgcgggg
     8101 atctcatgct ggagttcttc gcccacccca acttgtttat tgcagcttat aatggttaca
     8161 aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt
     8221 gtggtttgtc caaactcatc aatgtatctt atcatgtctg tataccgtcg acctctagct
     8281 agagcttggc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa
     8341 ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga
     8401 gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt
     8461 gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct
     8521 cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat
     8581 cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga
     8641 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt
     8701 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt
     8761 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc
     8821 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa
     8881 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct
     8941 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta
     9001 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg
     9061 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc
     9121 ctaactacgg ctacactaga aggacagtat ttggtatctg cgctctgctg aagccagtta
     9181 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg
     9241 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt
     9301 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg
     9361 tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta
     9421 aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg
     9481 aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg
     9541 tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc
     9601 gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg
     9661 agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg
     9721 aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag
     9781 gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat
     9841 caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc
     9901 cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc
     9961 ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa
    10021 ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac
    10081 gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt
    10141 cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc
    10201 gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa
    10261 caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca
    10321 tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat
    10381 acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa
    10441 aagtgccacc tgacgtc
//
