LOCUS       sequence-91613-e        9161 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  FAK fret control sensor without FERM domain with 30 nm linker.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9161)
  AUTHORS   Ritt M, Guan JL, Sivaramakrishnan S
  TITLE     Visualizing and manipulating focal adhesion kinase regulation in 
            live cells.
  JOURNAL   J Biol Chem. 2013 Mar 29;288(13):8875-86. doi: 
            10.1074/jbc.M112.421164. Epub 2013 Feb 7.
  PUBMED    23393139
REFERENCE   2  (bases 1 to 9161)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.M112.421164"; journalName: "J Biol Chem"; date: 
            "2013-03-29- 29"; volume: "288"; issue: "13"; pages: "8875-86"
COMMENT     Sequence Label: pCDNA-FRT-FAK-kinase30
FEATURES             Location/Qualifiers
     source          1..9161
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     863..882
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        863..881
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             920..943
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             join(962..964,965..1675)
                     /codon_start=1
                     /product="enhanced variant of YFP (Heikal et al., 2001)"
                     /label=Citrine
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTFGYGLMCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     CDS             join(962..964,965..1675)
                     /codon_start=1
                     /product="enhanced YFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEYFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTFGYGLMCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     CDS             1700..1720
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             join(2375..2377,2378..3088)
                     /codon_start=1
                     /product="enhanced monomeric variant of CFP (Rizzo et al., 
                     2004)"
                     /label=mCerulean
                     /note=" mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNAISDNVYITADKQKNGIKA
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(5113..5130)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5119..5343
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(5476..5495)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     protein_bind    5627..5674
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     CDS             5682..6704
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     CDS             5682..6704
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     polyA_signal    6834..6955
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6871..6890)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6925..6944
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7004..7020)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7004..7020)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7017..7039)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7028..7044
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7052..7058,7059..7076,7077..7082))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7097..7118
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7235..7252)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7406..7994)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7486..7505)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8165..8956,8957..9025))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8788..8807
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9026..9130)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc
      901 gtttaaactt aagcttatgg actacaagga cgatgacgac aagggaagcg gaggaagcgg
      961 agtgagcaag ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg agctggacgg
     1021 cgacgtaaac ggccacaagt tcagcgtgtc cggcgagggc gagggcgatg ccacctacgg
     1081 caagctgacc ctgaagttca tctgcaccac cggcaagctg cccgtgccct ggcccaccct
     1141 cgtgaccacc ttcggctacg gcctgatgtg cttcgcccgc taccccgacc acatgaagca
     1201 gcacgacttc ttcaagtccg ccatgcccga aggctacgtc caggagcgca ccatcttctt
     1261 caaggacgac ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg acaccctggt
     1321 gaaccgcatc gagctgaagg gcatcgactt caaggaggac ggcaacatcc tggggcacaa
     1381 gctggagtac aactacaaca gccacaacgt ctatatcatg gccgacaagc agaagaacgg
     1441 catcaaggtg aacttcaaga tccgccacaa catcgaggac ggcagcgtgc agctcgccga
     1501 ccactaccag cagaacaccc ccatcggcga cggccccgtg ctgctgcccg acaaccacta
     1561 cctgagctac cagtccaaac tgagcaaaga ccccaacgag aagcgcgatc acatggtcct
     1621 gctggagttc gtgaccgccg ccgggatcac tctcggcatg gacgagctgt acaaggaatt
     1681 cggaagcgga ggaagcggag aaaacctgta ttttcagggc ggaagcggag aagaggaaga
     1741 gaagaagaaa gaagaggaag aaaagaaaca aaaagaagaa caagaaagac ttgcaaaaga
     1801 agaggcagag agaaaacaaa aagaagaaca agaaagactt gcaaaagaag aggcagagag
     1861 aaaacaaaag gaggaagaag agagaaaaca aaaggaagaa gaagagagaa aacaaaagga
     1921 ggaagaagaa agaaaattaa aggaggaaca agaaagaaaa gctgcagaag aaaagaaagc
     1981 taaagaagaa gctgagagaa aggctaaaga agaacaagaa aggaaagctg aagaagagag
     2041 aaagaagaaa gaagaggaag aaagacttga aagagaaaga aaagagagag aagaacaaga
     2101 aaagaaagcc aaagaagagg cagagagaat tgcaaagtta gaggctgaaa agaaggcaga
     2161 agaagaaaga aaagccaaag aagaagaaga gagaaaagcc aaagaagaag aggaaagaaa
     2221 gaagaaagag gagcaagaaa gacttgcaaa agaaaaggaa gaagcagaaa gaaaagctgc
     2281 agaggaaaag aaagctaaag aagaacaaga aagaaaagaa aaggaagaag cagaaagaaa
     2341 acaaagaggc tctggcggct ctggcggcgc gcccgtgagc aagggcgagg agctgttcac
     2401 cggggtggtg cccatcctgg tcgagctgga cggcgacgta aacggccaca agttcagcgt
     2461 gtccggcgag ggcgagggcg atgccaccta cggcaagctg accctgaagt tcatctgcac
     2521 caccggcaag ctgcccgtgc cctggcccac cctcgtgacc accctgacct ggggcgtgca
     2581 gtgcttcgcc cgctaccccg accacatgaa gcagcacgac ttcttcaagt ccgccatgcc
     2641 cgaaggctac gtccaggagc gcaccatctt cttcaaggac gacggcaact acaagacccg
     2701 cgccgaggtg aagttcgagg gcgacaccct ggtgaaccgc atcgagctga agggcatcga
     2761 cttcaaggag gacggcaaca tcctggggca caagctggag tacaacgcca tcagcgacaa
     2821 cgtctatatc accgccgaca agcagaagaa cggcatcaag gccaacttca agatccgcca
     2881 caacatcgag gacggcagcg tgcagctcgc cgaccactac cagcagaaca cccccatcgg
     2941 cgacggcccc gtgctgctgc ccgacaacca ctacctgagc acccagtcca agctgagcaa
     3001 agaccccaac gagaagcgcg atcacatggt cctgctggag ttcgtgaccg ccgccgggat
     3061 cactctcggc atggacgagc tgtacaagtt aattaaggga agcggaggaa gcggaggaag
     3121 cggaggaagc ggaccatcaa ccagagatta tgaaattcaa agggagagaa ttgaactggg
     3181 gcgctgcatt ggtgaaggac agtttggaga tgtgcaccaa ggaatttaca tgagtccgga
     3241 aaatccagct atggctgtag caatcaaaac atgtaaaaac tgcacctcag acagcgttag
     3301 agaaaagttc ctacaagaag ccttaacaat gcgtcagttt gatcatcctc acattgtgaa
     3361 gctcattgga gttattacag aaaacccagt gtggataatc atggagctct gtacacttgg
     3421 agagttgaga tcgtttctgc aagtaagaaa attcagcttg gacctggcct ccctcatcct
     3481 ctacgcttac cagcttagca cagcacttgc ttacctagag agcaaaagat ttgtacatag
     3541 agatattgct gctaggaacg tgctggtatc tgccactgac tgtgtgaaat tgggtgactt
     3601 tggcttatcc cgatacatgg aagacagtac ttactataag gcgtccaaag gaaagttacc
     3661 tatcaaatgg atggctccag agtcaatcaa cttccgacgg tttacctcag caagcgatgt
     3721 gtggatgttt ggtgtgtgta tgtgggagat cctgatgcat ggggtaaagc ccttccaggg
     3781 agtgaaaaat aatgatgtta ttggtcggat tgagaacggt gagcggctcc ccatgcctcc
     3841 gaactgccct cccaccctct acagccttat gaccaagtgc tgggcatacg accctagtag
     3901 acgacccagg tttactgaac ttaaagcaca actcagtaca atactggagg aggagaagct
     3961 gcagcaagag gaacgaatga gaatggaatc caggcgacaa gtcacagtat cctgggactc
     4021 aggaggatca gatgaagctc ctcccaagcc cagcaggcct ggttacccca gcccaaggtc
     4081 cagtgaaggg ttttatccga gtcctcagca tatggtacag ccaaatcact accaggtatc
     4141 tggctactct ggttctcatg ggataccagc catggcaggc agcatttatc ctgggcaggc
     4201 gtctctcttg gatcaaacag attcctggaa ccatcgacct caggaagtat cagcatggca
     4261 gccaaacatg gaggattcgg gcactttgga tgtacgagga atggggcagg ttctgcccac
     4321 acatctcatg gaggagaggt taataagaca acagcaagag atggaagaag atcaacgctg
     4381 gcttgagaaa gaggaacgat tcctggtaat gaaacctgat gtgcggctct ccagaggcag
     4441 cattgaacgg gaggacggag gtctccaggg cccagctggt aaccagcaca tatatcagcc
     4501 tgtgggtaaa ccagatcatg ccgctccacc aaagaagccc cctcgccctg gagcccccca
     4561 cttgggcagc ctcgcgagcc tgaacagccc cgtggacagc tacaacgaag gcgtgaagat
     4621 caagccacag gaaatcagcc ctcctcctac ggccaacctg gaccgctcca atgacaaagt
     4681 ctatgagaat gtaaccgggc tggtgaaagc tgtcatagag atgtccagta aaatacagcc
     4741 agctccgcca gaggagtacg tgcccatggt aaaggaggtt ggcttggcgc tgagaacctt
     4801 gctagcaaca gtggatgagt cgctgccagt gcttcctgca agcacccaca gagagattga
     4861 gatggcccag aaactgctga actctgacct ggctgagctc attaacaaga tgaagctggc
     4921 ccagcagtac gtcatgacca gcctgcagca ggagtacaag aagcaaatgc tgacggctgc
     4981 tcacgctctg gctgtggatg ccaagaactt gctggatgtc atcgatcaag ccagactgaa
     5041 aatgatcagc cagtccaggc cccactgagc ggccgctcga gtctagaggg cccgtttaaa
     5101 cccgctgatc agcctcgact gtgccttcta gttgccagcc atctgttgtt tgcccctccc
     5161 ccgtgccttc cttgaccctg gaaggtgcca ctcccactgt cctttcctaa taaaatgagg
     5221 aaattgcatc gcattgtctg agtaggtgtc attctattct ggggggtggg gtggggcagg
     5281 acagcaaggg ggaggattgg gaagacaata gcaggcatgc tggggatgcg gtgggctcta
     5341 tggcttctga ggcggaaaga accagctggg gctctagggg gtatccccac gcgccctgta
     5401 gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca
     5461 gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct
     5521 ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt gctttacggc
     5581 acctcgaccc caaaaaactt gattagggtg atggttcacg tacctagaag ttcctattcc
     5641 gaagttccta ttctctagaa agtataggaa cttccttggc caaaaagcct gaactcaccg
     5701 cgacgtctgt cgagaagttt ctgatcgaaa agttcgacag cgtctccgac ctgatgcagc
     5761 tctcggaggg cgaagaatct cgtgctttca gcttcgatgt aggagggcgt ggatatgtcc
     5821 tgcgggtaaa tagctgcgcc gatggtttct acaaagatcg ttatgtttat cggcactttg
     5881 catcggccgc gctcccgatt ccggaagtgc ttgacattgg ggaattcagc gagagcctga
     5941 cctattgcat ctcccgccgt gcacagggtg tcacgttgca agacctgcct gaaaccgaac
     6001 tgcccgctgt tctgcagccg gtcgcggagg ccatggatgc gatcgctgcg gccgatctta
     6061 gccagacgag cgggttcggc ccattcggac cgcaaggaat cggtcaatac actacatggc
     6121 gtgatttcat atgcgcgatt gctgatcccc atgtgtatca ctggcaaact gtgatggacg
     6181 acaccgtcag tgcgtccgtc gcgcaggctc tcgatgagct gatgctttgg gccgaggact
     6241 gccccgaagt ccggcacctc gtgcacgcgg atttcggctc caacaatgtc ctgacggaca
     6301 atggccgcat aacagcggtc attgactgga gcgaggcgat gttcggggat tcccaatacg
     6361 aggtcgccaa catcttcttc tggaggccgt ggttggcttg tatggagcag cagacgcgct
     6421 acttcgagcg gaggcatccg gagcttgcag gatcgccgcg gctccgggcg tatatgctcc
     6481 gcattggtct tgaccaactc tatcagagct tggttgacgg caatttcgat gatgcagctt
     6541 gggcgcaggg tcgatgcgac gcaatcgtcc gatccggagc cgggactgtc gggcgtacac
     6601 aaatcgcccg cagaagcgcg gccgtctgga ccgatggctg tgtagaagta ctcgccgata
     6661 gtggaaaccg acgccccagc actcgtccga gggcaaagga atagcacgta ctacgagatt
     6721 tcgattccac cgccgccttc tatgaaaggt tgggcttcgg aatcgttttc cgggacgccg
     6781 gctggatgat cctccagcgc ggggatctca tgctggagtt cttcgcccac cccaacttgt
     6841 ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag
     6901 catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg
     6961 tctgtatacc gtcgacctct agctagagct tggcgtaatc atggtcatag ctgtttcctg
     7021 tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta
     7081 aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg
     7141 ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga
     7201 gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg
     7261 tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag
     7321 aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc
     7381 gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca
     7441 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt
     7501 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc
     7561 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc
     7621 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc
     7681 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact
     7741 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg
     7801 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaaggaca gtatttggta
     7861 tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca
     7921 aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa
     7981 aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg
     8041 aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc
     8101 ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg
     8161 acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat
     8221 ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg
     8281 gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa
     8341 taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca
     8401 tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc
     8461 gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt
     8521 cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa
     8581 aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat
     8641 cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct
     8701 tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga
     8761 gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag
     8821 tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga
     8881 gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca
     8941 ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg
     9001 cgacacggaa atgttgaata ctcatactct tcctttttca atattattga agcatttatc
     9061 agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag
     9121 gggttccgcg cacatttccc cgaaaagtgc cacctgacgt c
//
