LOCUS       sequence-91627-e        3166 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  ORF encoding p38 KTR mCerulean3 flanked by Gateway sequences.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3166)
  AUTHORS   Regot S, Hughey JJ, Bajar BT, Carrasco S, Covert MW
  TITLE     High-sensitivity measurements of multiple kinase activities in live 
            single cells.
  JOURNAL   Cell. 2014 Jun 19;157(7):1724-34. doi: 10.1016/j.cell.2014.04.039.
  PUBMED    24949979
REFERENCE   2  (bases 1 to 3166)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2014.04"; journalName: "Cell"; date: "2014-06-19- 
            19"; volume: "157"; issue: "7"; pages: "1724-34"
COMMENT     Sequence Label: pENTR-p38KTRmCerulean3
FEATURES             Location/Qualifiers
     source          1..3166
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      103..189
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      281..308
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     primer_bind     316..339
                     /label=pENTR-F
                     /note="pENTR vectors, forward primer"
     protein_bind    358..457
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             join(652..654,655..657,658..1371)
                     /codon_start=1
                     /product="enhanced monomeric variant of CFP (Rizzo et al., 
                     2004)"
                     /label=mCerulean
                     /note=" mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLSWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNAIHGNVYITADKQKNGIK
                     ANFGLNCNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(697..718)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(958..977)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1305..1326
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     protein_bind    complement(1395..1494)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1606..1625)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             1617..2426
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVSLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(1684..1703)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      2516..3104
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3005..3024
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgctagca tggatctcgg
       61 ggacgtctaa ctactaagcg agagtaggga actgccaggc atcaaataaa acgaaaggct
      121 cagtcggaag actgggcctt tcgttttatc tgttgtttgt cggtgaacgc tctcctgagt
      181 aggacaaatc cgccgggagc ggatttgaac gttgtgaagc aacggcccgg agggtggcgg
      241 gcaggacgcc cgccataaac tgccaggcat caaactaagc agaaggccat cctgacggat
      301 ggcctttttg cgtttctaca aactcttcct gttagttagt tacttaagct cgggccccaa
      361 ataatgattt tattttgact gatagtgacc tgttcgttgc aacaaattga taagcaatgc
      421 ttttttataa tgccaacttt gtacaaaaaa gcaggctcca ccatgcgtaa gccagatctc
      481 cgcgttctta tcccacctgg cagcaagaac acgatgccat cagtgaatca aaggataaat
      541 aactccaagc gctcggccgg tctcaacgaa gacgagtcgc catctaagaa gctggcgtcg
      601 ccggaggtgt cgtcaaggct ggagcgcctg accctccagt ccagcactag tatggtgagc
      661 aagggcgagg agctgttcac cggggtggtg cccatcctgg tcgagctgga cggcgacgta
      721 aacggccaca agttcagcgt gtccggcgag ggcgagggcg atgccaccta cggcaagctg
      781 accctgaagt tcatctgcac caccggcaag ctgcccgtgc cctggcccac cctcgtgacc
      841 accctgagct ggggcgtgca gtgcttcgcc cgctaccccg accacatgaa gcagcacgac
      901 ttcttcaagt ccgccatgcc cgaaggctac gtccaggagc gcaccatctt cttcaaggac
      961 gacggcaact acaagacccg cgccgaggtg aagttcgagg gcgacaccct ggtgaaccgc
     1021 atcgagctga agggcatcga cttcaaggag gacggcaaca tcctggggca caagctggag
     1081 tacaacgcca tccacggcaa cgtctatatc accgccgaca agcagaagaa cggcatcaag
     1141 gccaacttcg gcctgaactg caacatcgag gacggcagcg tgcagctcgc cgaccactac
     1201 cagcagaaca cccccatcgg cgacggcccc gtgctgctgc ccgacaacca ctacctgagc
     1261 acccagtcca agctgagcaa agaccccaac gagaagcgcg atcacatggt cctgctggag
     1321 ttcgtgaccg ccgccgggat cactctcggc atggacgagc tgtacaagta agcggccgca
     1381 ctcgagatat ctagacccag ctttcttgta caaagttggc attataagaa agcattgctt
     1441 atcaatttgt tgcaacgaac aggtcactat cagtcaaaat aaaatcatta tttgccatcc
     1501 agctgcagct ctggcccgtg tctcaaaatc tctgatgtta cattgcacaa gataaaaata
     1561 tatcatcatg aacaataaaa ctgtctgctt acataaacag taatacaagg ggtgttatga
     1621 gccatattca acgggaaacg tcgaggccgc gattaaattc caacatggat gctgatttat
     1681 atgggtataa atgggctcgc gataatgtcg ggcaatcagg tgcgacaatc tatcgcttgt
     1741 atgggaagcc cgatgcgcca gagttgtttc tgaaacatgg caaaggtagc gttgccaatg
     1801 atgttacaga tgagatggtc agactaaact ggctgacgga atttatgcct cttccgacca
     1861 tcaagcattt tatccgtact cctgatgatg catggttact caccactgcg atccccggaa
     1921 aaacagcatt ccaggtatta gaagaatatc ctgattcagg tgaaaatatt gttgatgcgc
     1981 tggcagtgtc cctgcgccgg ttgcattcga ttcctgtttg taattgtcct tttaacagcg
     2041 atcgcgtatt tcgtctcgct caggcgcaat cacgaatgaa taacggtttg gttgatgcga
     2101 gtgattttga tgacgagcgt aatggctggc ctgttgaaca agtctggaaa gaaatgcata
     2161 aacttttgcc attctcaccg gattcagtcg tcactcatgg tgatttctca cttgataacc
     2221 ttatttttga cgaggggaaa ttaataggtt gtattgatgt tggacgagtc ggaatcgcag
     2281 accgatacca ggatcttgcc atcctatgga actgcctcgg tgagttttct ccttcattac
     2341 agaaacggct ttttcaaaaa tatggtattg ataatcctga tatgaataaa ttgcagtttc
     2401 atttgatgct cgatgagttt ttctaatcag aattggttaa ttggttgtaa cattattcag
     2461 attgggcccc gttccactga gcgtcagacc cggtagaaaa gatcaaagga tcttcttgag
     2521 atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg
     2581 tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca
     2641 gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga
     2701 actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca
     2761 gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc
     2821 agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca
     2881 ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa
     2941 aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc
     3001 cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc
     3061 gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg
     3121 cctttttacg gttcctggcc ttttgctggc cttttgctca catgtt
//
