LOCUS       sequence-91646-e        1134 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Fluorescent membrane voltage sensor.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1134)
  AUTHORS   Zou P, Zhao Y, Douglass AD, Hochbaum DR, Brinks D, Werley CA, 
            Harrison DJ, Campbell RE, Cohen AE
  TITLE     Bright and fast multicoloured voltage reporters via electrochromic 
            FRET.
  JOURNAL   Nat Commun. 2014 Aug 13;5:4625. doi: 10.1038/ncomms5625.
  PUBMED    25118186
REFERENCE   2  (bases 1 to 1134)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2014-08-13"; volume: "5:4625. doi"; pages: " 
            10.1038/ncomms5625"
COMMENT     Sequence Label: FCK-QuasAr2-Citrine
FEATURES             Location/Qualifiers
     source          1..1134
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(54..75)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(315..334)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     CDS             474..725
                     /codon_start=1
                     /product="C-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VC155
                     /translation="DKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH
                     YLSYQSALSKDPNEKRDHMVLLEFVTAAGITLGMDXLYK"
     primer_bind     662..683
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(890..910)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
ORIGIN
        1 cnnggnntnn tctgggcgac nccgagttga ggttcaccgg ggtggtgccc atcctggtcg
       61 agctggacgg cgacgtaaac ggccacaagt tcagcgtgtc cggcgagggc gagggcgatg
      121 ccacctacgg caagctgacc ctgaagttca tctgcaccac cggcaagctg cccgtgccct
      181 ggcccaccct cgtgaccacc ttcggctacg gcctgatgtg cttcgcccgc taccccgacc
      241 acatgaagca gcacgacttc ttcaagtccg ccatgcccga aggctacgtc caggagcgca
      301 ccatcttctt caaggacgac ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg
      361 acaccctggt gaaccgcatc gagctgaagg gcatcgactt caaggaggac ggcaacatcc
      421 tggggcacaa gctggagtac aactacaaca gccacaacgt ctatatcatg gccgacaagc
      481 agaagaacgg catcaaggtg aacttcaaga tccgccacaa catcgaggac ggcagcgtgc
      541 agctcgccga ccactaccag cagaacaccc ccatcggcga cggccccgtg ctgctgcccg
      601 acaaccacta cctgagctac cagtccgccc tgagcaaaga ccccaacgag aagcgcgatc
      661 acatggtcct gctggagttc gtgaccgccg ccgggatcac tctcggcatg gacganctgt
      721 acaagagtag aatcacaagc gaangcgagt acatccccct ggatcaaata gacataaatg
      781 taggtggatt ttgttatgag aatgaagtat aagaattcga tatcaagctt atcgataatc
      841 aacctctgga ttacaaaatt tgtgaaagat tgactggtat tcttaactat gttgctcctt
      901 ttacgctatg tggatacgct gctttaatgc ctttgtatca tgctattgct tcccgtatgg
      961 ctttcatttt ctcctccttg tataaatcct ggnngctgtc tctttatgan nnttgtggcc
     1021 cgttgtcnnn angtnnnnnn gngnactnng tttgctgann caaccccnnn nggnngggca
     1081 ttnccaccac ctgncagcnn ctttnngggn ntttnnnntt ncccnnnccc nant
//
