LOCUS       sequence-91662-e         849 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Retroviral expression of DsRed in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 849)
  AUTHORS   Yen HC, Xu Q, Chou DM, Zhao Z, Elledge SJ
  TITLE     Global protein stability profiling in mammalian cells.
  JOURNAL   Science. 2008 Nov 7;322(5903):918-23. doi: 10.1126/science.1160489.
  PUBMED    18988847
REFERENCE   2  (bases 1 to 849)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1126/science"; 
            journalName: "Science"; date: "2008-11-7- 7"; volume: "322"; issue: 
            "5903"; pages: "918-23"
COMMENT     Sequence Label: MSCV-CMV-DsRed-IRES-d4EGFP
FEATURES             Location/Qualifiers
     source          1..849
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(178..849)
                     /codon_start=1
                     /product="rapidly maturing tetrameric variant of DsRed 
                     fluorescent protein (Bevis and Glick, 2002)"
                     /label=DsRed-Express
                     /note="mammalian codon-optimized"
                     /translation="SSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAKLK
                     VTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWERVMNFEDGGVVT
                     VTQDSSLQDGSFIYKVKFIGVNFPSDGPVMQKKTMGWEASTERLYPRDGVLKGEIHKAL
                     KLKDGGHYLVEFKSIYMAKKPVQLPGYYYVDSKLDITSHNEDYTIVEQYERAEGRHHLF
                     L"
     primer_bind     complement(240..263)
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     primer_bind     complement(438..457)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     655..675
                     /label=DsRed1-N
                     /note="DsRed1, reverse primer"
     primer_bind     707..725
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
ORIGIN
        1 acagggccag gtttccgggc cctcacattg ccaaaagacg gcaatatggt ggaaaataac
       61 atatagacaa acgcacaccg gccttattcc aagcggcttc ggccagtaac gttagggggg
      121 ggggagggag aggggcggat cccgggcccg cggtaccgtc gactgcagaa ttggccgcta
      181 caggaacagg tggtggcggc cctcggcgcg ctcgtactgc tccacgatgg tgtagtcctc
      241 gttgtgggag gtgatgtcca gcttggagtc cacgtagtag tagccgggca gctgcacggg
      301 cttcttggcc atgtagatgg acttgaactc caccaggtag tggccgccgt ccttcagctt
      361 cagggccttg tggatctcgc ccttcagcac gccgtcgcgg gggtacaggc gctcggtgga
      421 ggcctcccag cccatagtct tcttctgcat tacggggccg tcggagggga agttcacgcc
      481 gatgaacttc accttgtaga tgaaggagcc gtcctgcagg gaggagtcct gggtcacggt
      541 caccacgccg ccgtcctcga agttcatcac gcgctcccac ttgaagccct cggggaagga
      601 cagcttcttg tagtcgggga tgtcggcggg gtgcttcacg tacaccttgg agccgtactg
      661 gaactggggg gacaggatgt cccaggcgaa gggcaggggg ccgcccttgg tcaccttcag
      721 cttggcggtc tgggtgccct cgtaggggcg gccctcgccc tcgccctcga tctcgaactc
      781 gtggccgttc acggagccct ccatgcgcac cttgaagcgc atgaactcct tgatgacgtc
      841 ctcggagga
//
