LOCUS       sequence-91664-e         881 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  This plasmid can be used to mutate genes in Toxoplasma gondii using 
            the CRISPR/Cas9 system after having an appropriate protospacer 
            cloned into it..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 881)
  TITLE     Lourido lab Toxoplasma CRISPR Plasmid
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 881)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pU6-Universal
FEATURES             Location/Qualifiers
     source          1..881
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_RNA        631..706
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
ORIGIN
        1 ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac
       61 gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatggga
      121 tgagacaaag tgcgcgagtt gaaatcgtcg tggggacgat ttcaccgcgg ccacatgttg
      181 gagacactga gggcacacgg gaaacgcgaa agatttcaaa ttaacgtacc caaacgcgaa
      241 agcttgcgca gcatacactc gaagcgaaca tcccgaacca tcgagaggcg gagagcgata
      301 agtctttcac gctgcgaagt gttgcgacgg ctgcgccgct gcactgtgaa ttgggcgcca
      361 atattgcatc ctaggcctga cgcgcctcct gcagaacgcg agacactggg atatgtagag
      421 ccaaggggga aaccttcgaa ctctcgaatg tcttctctga caagaatcat atttccatca
      481 gttctgtcag attttcaaat ggcgacctgc agaggcctgc ttcctccctg tgcgctcttc
      541 gaaggggctt tctgtcgcgc agggtcacct cgtccccgaa gggggtgttt gccttctggt
      601 aaatggggat gtcaagttga gaccggtctc gttttagagc tagaaatagc aagttaaaat
      661 aaggctagtc cgttatcaac ttgaaaaagt ggcaccgagt cggtgctttt tttttctttt
      721 tctctagagg taccatgcat gtcccgcgtt cgtgaaattc tctgcatcag cggagtgatc
      781 aggaatcatc gtctcagcgg gatgacgttg cggagcaggc cggctcgcgg tgggcagtca
      841 gatgccgaag gcgtaactca ggacggcttg cgctcatcgc a
//
