LOCUS       sequence-91668-e         871 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Solubly expresses monomeric streptavidin containing S25H mutation in
            E. coli as an MBP fusion. App Microbiol.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 871)
  AUTHORS   Demonte D, Dundas CM, Park S
  TITLE     Expression and purification of soluble monomeric streptavidin in 
            Escherichia coli.
  JOURNAL   Appl Microbiol Biotechnol. 2014;98(14):6285-95. doi: 
            10.1007/s00253-014-5682-y. Epub 2014 Apr 2.
  PUBMED    24691867
REFERENCE   2  (bases 1 to 871)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Appl 
            Microbiol Biotechnol."; date: "2014"; volume: "98(14):6285-95. doi";
            pages: " 10.1007/s00253-014-5682-y. Epub 2014 Apr 2"
COMMENT     Sequence Label: pET-MBP-mSA2
FEATURES             Location/Qualifiers
     source          1..871
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             34..51
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             61..78
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
ORIGIN
        1 actttaagaa ggagatatac catgggcagc agccatcatc atcatcatca cagcagcggc
       61 ctggtgccgc gcggcagcca tatgaaaatc gaagaaggta aactggtaat ctggattaac
      121 ggcgataaag gctataacgg tctcgctgaa gtcggtaaga aattcgagaa agataccgga
      181 attaaagtca ccgttgagca tccggataaa ctggaagaga aattcccaca ggttgcggca
      241 actggcgatg gccctgacat tatcttctgg gcacacgacc gctttggtgg ctacgctcaa
      301 tctggcctgt tggctgaaat caccccggac aaagcgttcc aggacaagct gtatccgttt
      361 acctgggatg ccgtacgtta caacggcaag ctgattgctt acccgatcgc tgttgaagcg
      421 ttatcgctga tttataacaa agatctgctg ccgaacccgc caaaaacctg ggaagagatc
      481 ccggcgctgg ataaagaact gaaagcgaaa ggtaagagcg cgctgatgtt caacctgcaa
      541 gaaccgtact tcacctggcc gctgattgct gctgacgggg gttatgcgtt caagtatgaa
      601 aacggcaagt acgacattaa agacgtgggc gtggataacg ctggcgcgaa agcgggtctg
      661 accttcctgg ttgacctgat taaaaacaaa cacatgaatg cagacaccga ttactccatc
      721 gcagaagctg cctttaataa aggcgaaaca gcgatgacca tcaacggccc gtgggcatgg
      781 tccaacatcg acaccagcaa agtgaattat ggtgtaacgg tactgccgac cttcaagggt
      841 caaccatcca aaccgttcgt tggcgtgctg a
//
