LOCUS       sequence-91670-e         850 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Solubly expresses monomeric streptavidin containing S25H mutation in
            E. coli as an MBP fusion. App Microbiol.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 850)
  AUTHORS   Demonte D, Dundas CM, Park S
  TITLE     Expression and purification of soluble monomeric streptavidin in 
            Escherichia coli.
  JOURNAL   Appl Microbiol Biotechnol. 2014;98(14):6285-95. doi: 
            10.1007/s00253-014-5682-y. Epub 2014 Apr 2.
  PUBMED    24691867
REFERENCE   2  (bases 1 to 850)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Appl 
            Microbiol Biotechnol."; date: "2014"; volume: "98(14):6285-95. doi";
            pages: " 10.1007/s00253-014-5682-y. Epub 2014 Apr 2"
COMMENT     Sequence Label: pET-MBP-mSA2
FEATURES             Location/Qualifiers
     source          1..850
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(21..38)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(48..71)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             complement(93..434)
                     /codon_start=1
                     /product="monomeric streptavidin/rhizavidin hybrid (Lim et 
                     al., 2013)"
                     /label=mSA
                     /translation="AEAGITGTWYNQHGSTFTVTAGADGNLTGQYENRAQGTGCQNSPY
                     TLTGRYNGTKLEWRVEWNNSTENCHSRTEWRGQYQGGAEARINTQWNLTYEGGSGPATE
                     QGQDTFTKVK"
     CDS             complement(435..455)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             complement(477..488)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(540..563)
                     /label=MBP-F
                     /note="Maltose binding protein, forward primer"
     regulatory      671..680
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 ttgttagcag ccggatctca gtggtggtgg tggtggtgct cgagttactt gtcgtcatcg
       61 tccttgtagt cggatccaga cgccgcagac ggtttaactt tggtgaaggt gtcctgaccc
      121 tgttcggtcg ccggaccaga accaccttcg taggtcaggt tccactgggt gttgatacgc
      181 gcttccgcac caccctggta ctgaccacgc cattcggtac gagagtggca gttttcggta
      241 gagttgttcc attcaacacg ccattccagt ttggtaccgt tgtaacgacc ggtcagggtg
      301 tacggagagt tctggcaacc agtgccctgc gcacggtttt cgtactgacc ggtcaggtta
      361 ccgtccgcac ccgcggtaac ggtgaaggta gaaccatgct ggttgtacca cgtgccggtg
      421 atacccgctt ccgcgccctg gaaatacagg ttctcgctag cgaattcgga tcctcccctt
      481 ccctcgatcc cgaggttgtt gttattgtta ttgttgttgt tgttcgagct cgaattagtc
      541 tgcgcgtctt tcagggcttc atcgacagtc tgacgaccgc tggcggcgtt gatcaccgca
      601 gtacgcacgg cataccagaa agcggacatc tgcgggatgt tcggcatgat ttcacctttc
      661 tgggcgtttt ccatggtggc ggcaatacgt ggatctttcg ccaactcttc ctcgtaagac
      721 ttcagcgcta cggcacccag cggtttgtct ttattaaccg cttccagacc ttcatcagtc
      781 agcagatagt tttcgaggaa ctcttttgcc agctctttgt tcggactggc ggcgttaata
      841 cctgcgctca
//
