LOCUS       sequence-91671-e         910 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Solubly expresses monomeric streptavidin containing S25H mutation in
            E. coli as a thioredoxin fusion. App Microbiol.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 910)
  AUTHORS   Demonte D, Dundas CM, Park S
  TITLE     Expression and purification of soluble monomeric streptavidin in 
            Escherichia coli.
  JOURNAL   Appl Microbiol Biotechnol. 2014;98(14):6285-95. doi: 
            10.1007/s00253-014-5682-y. Epub 2014 Apr 2.
  PUBMED    24691867
REFERENCE   2  (bases 1 to 910)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Appl 
            Microbiol Biotechnol."; date: "2014"; volume: "98(14):6285-95. doi";
            pages: " 10.1007/s00253-014-5682-y. Epub 2014 Apr 2"
COMMENT     Sequence Label: pET-Trx-mSA2
FEATURES             Location/Qualifiers
     source          1..910
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             23..349
                     /codon_start=1
                     /gene="trxA"
                     /product="E. coli thioredoxin"
                     /label=TrxA
                     /translation="MSDKIIHLTDDSFDTDVLKADGAILVDFWAEWCGPCKMIAPILDE
                     IADEYQGKLTVAKLNIDQNPGTAPKYGIRGIPTLLLFKNGEVAATKVGALSKGQLKEFL
                     DANLA"
     CDS             371..388
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             398..415
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             422..466
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     CDS             482..496
                     /codon_start=1
                     /product="enterokinase recognition and cleavage site"
                     /label=enterokinase site
                     /translation="DDDDK"
     CDS             530..550
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             551..892
                     /codon_start=1
                     /product="monomeric streptavidin/rhizavidin hybrid (Lim et 
                     al., 2013)"
                     /label=mSA
                     /translation="AEAGITGTWYNQHGSTFTVTAGADGNLTGQYENRAQGTGCQNSPY
                     TLTGRYNGTKLEWRVEWNNSTENCHSRTEWRGQYQGGAEARINTQWNLTYEGGSGPATE
                     QGQDTFTKVK"
ORIGIN
        1 actttaagaa ggagatatac atatgagcga taaaattatt cacctgactg acgacagttt
       61 tgacacggat gtactcaaag cggacggggc gatcctcgtc gatttctggg cagagtggtg
      121 cggtccgtgc aaaatgatcg ccccgattct ggatgaaatc gctgacgaat atcagggcaa
      181 actgaccgtt gcaaaactga acatcgatca aaaccctggc actgcgccga aatatggcat
      241 ccgtggtatc ccgactctgc tgctgttcaa aaacggtgaa gtggcggcaa ccaaagtggg
      301 tgcactgtct aaaggtcagt tgaaagagtt cctcgacgct aacctggccg gttctggttc
      361 tggccatatg caccatcatc atcatcattc ttctggtctg gtgccacgcg gttctggtat
      421 gaaagaaacc gctgctgcta aattcgaacg ccagcacatg gacagcccag atctgggtac
      481 cgacgacgac gacaaggcca tggctgatat cggatccgaa ttcgctagcg agaacctgta
      541 tttccagggc gcggaagcgg gtatcaccgg cacgtggtac aaccagcatg gttctacctt
      601 caccgttacc gcgggtgcgg acggtaacct gaccggtcag tacgaaaacc gtgcgcaggg
      661 cactggttgc cagaactctc cgtacaccct gaccggtcgt tacaacggta ccaaactgga
      721 atggcgtgtt gaatggaaca actctaccga aaactgccac tctcgtaccg aatggcgtgg
      781 tcagtaccag ggtggtgcgg aagcgcgtat caacacccag tggaacctga cctacgaagg
      841 tggttctggt ccggcgaccg aacagggtca ggacaccttc accaaagtta aaccgtctgc
      901 ggcgtctgga
//
