LOCUS       sequence-91675-e         575 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  bacterial expression of human PITP alpha K61A.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 575)
  AUTHORS   Tilley SJ, Skippen A, Murray-Rust J, Swigart PM, Stewart A, Morgan 
            CP, Cockcroft S, McDonald NQ
  TITLE     Structure-function analysis of human [corrected] 
            phosphatidylinositol transfer protein alpha bound to 
            phosphatidylinositol.
  JOURNAL   Structure. 2004 Feb;12(2):317-26.
  PUBMED    14962392
REFERENCE   2  (bases 1 to 575)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Structure."; date: "2004-02"; volume: "12(2)"; pages: "317-26"
COMMENT     Sequence Label: pET14b-PITPalpha K61A
FEATURES             Location/Qualifiers
     source          1..575
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             27..44
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             54..71
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
ORIGIN
        1 gaaggagata taccatgggc agcagccatc atcatcatca tcacagcagc ggcctggtgc
       61 cgcgcggcag ccatatggtg ctgctcaagg agtatcgagt aatcctgcct gtgtctgtag
      121 atgagtatca agtggggcag ctgtattctg tggctgaggc cagtaaaaat gaaacgggtg
      181 gtggcgaagg cgtggaggtc ctggtgaatg agccctacga gaaggacggt gagaaaggcc
      241 agtacacaca cgcgatctac cacctgcaga gcaaagtacc cacgtttgtt cgaatgctgg
      301 ccccagaggg agccctgaat atacacgaga aagcctggaa tgcttacccc tactgcagaa
      361 ccgttattac aaatgagtac atgaaagaag actttctgat taaaattgaa acctggcaca
      421 aaccagatct tggcacgcag gagaatgtgc ataagctgga gcctgaggcg tggaaacacg
      481 tggaagccgt atatatagac attgcagatc gaagccaagt gctcagcaag gattacaagg
      541 cagaggaaga cccagcaaaa tttaaatcta tcaaa
//
