LOCUS       sequence-91682-e         860 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  3rd gen lentiviral Gateway destination vector, expression, CMV/TO 
            promoter, Hygro.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 860)
  AUTHORS   Campeau E, Ruhl VE, Rodier F, Smith CL, Rahmberg BL, Fuss JO, 
            Campisi J, Yaswen P, Cooper PK, Kaufman PD.
  TITLE     A versatile viral system for expression and depletion of proteins in
            mammalian cells.
  JOURNAL   PLoS One. 2009 Aug 6;4(8):e6529.
  PUBMED    19657394
REFERENCE   2  (bases 1 to 860)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2009-08-6"; volume: "4(8)"; pages: "e6529"
COMMENT     Sequence Label: pLenti CMV/TO Hygro DEST (693-2)
FEATURES             Location/Qualifiers
     source          1..860
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(join(97..103,104..121,122..127))
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     protein_bind    complement(152..276)
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    317..335
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    338..356
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     promoter        complement(358..561)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(387..407)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
ORIGIN
        1 gggatatatc aacggtggta tatccagtga tttttttctc cattttagct tccttagctc
       61 ctgaaaatct cgacggatcc taactcaaaa tccacacatt atacgagccg gaagcataaa
      121 gtgtaaagcc tggggtgcct aatgcggccg ccatagtgac tggatatgtt gtgttttaca
      181 gtattatgta gtctgttttt tatgcaaaat ctaatttaat atattgatat ttatatcatt
      241 ttacgtttct cgttcagctt ttttgtacaa acttgttgat atctgcagaa ttccaccaca
      301 ctggactagt cgacgatctc tatcactgat agggagatct ctatcactga tagggagagc
      361 tctgcttata tagacctccc accgtacacg cctaccgccc atttgcgtca atggggcgga
      421 gttgttacga cattttggaa agtcccgttg attttggtgc caaaacaaac tcccattgac
      481 gtcaatgggg tggagacttg gaaatccccg tgagtcaaac cgctatccac gcccattgat
      541 gtactgccaa aaccgcatca ccatggtaat agcgatgact aatacgtaga tgtactgcca
      601 agtaggaaag tcccataagg tcatgtactg ggcataatgc caggcgggcc atttaccgtc
      661 attgacgtca atagggggcg tacttggcat atgatacact tgatgtactg ccaagtgggc
      721 agtttaccgt aaatactcca cccattgacg tcaatggaaa gtccctattg gcgttactat
      781 gggaacatac gtcattattg acgtcaatgg gcgggggtcg ttgggcggtc agccaggcgg
      841 gccatttacc gtaagttatg
//
