LOCUS       sequence-91684-e         812 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  bacterial expression of PITP beta (phosphatidylinositol transfer 
            protein beta).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 812)
  AUTHORS   Shadan S, Holic R, Carvou N, Ee P, Li M, Murray-Rust J, Cockcroft S
  TITLE     Dynamics of lipid transfer by phosphatidylinositol transfer proteins
            in cells.
  JOURNAL   Traffic. 2008 Sep;9(10):1743-56. doi: 
            10.1111/j.1600-0854.2008.00794.x. Epub 2008 Jul 9.
  PUBMED    18636990
REFERENCE   2  (bases 1 to 812)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1111/j.1600-0854.2008.00794.x"; journalName: "Traffic"; date: 
            "2008-09"; volume: "9"; issue: "10"; pages: "1743-56"
COMMENT     Sequence Label: pRSETC-PITPbeta (splice variant 1)
FEATURES             Location/Qualifiers
     source          1..812
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             6..28
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             48..65
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             69..101
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             join(105..113,114..128)
                     /codon_start=1
                     /product="Xpress(TM) epitope tag, including an enterokinase
                     recognition and cleavage site"
                     /label=Xpress(TM) tag
                     /translation="DLYDDDDK"
ORIGIN
        1 ataattttgt ttaactttaa gaaggagata tacatatgcg gggttctcat catcatcatc
       61 atcatggtat ggctagcatg actggtggac agcaaatggg tcgggatctg tacgacgatg
      121 acgataagga tccgatggtg ctgatcaagg agttccgtgt ggttttgcca tgttctgttc
      181 aggagtatca ggttgggcag ctttactctg ttgcagaagc tagtaagaat gagactggtg
      241 gtggagaagg aattgaagtc ttaaagaatg aaccttatga gaaggatgga gaaaagggac
      301 agtatacgca caaaatttat cacctaaaga gcaaagtgcc tgcattcgtg aggatgattg
      361 ctcccgaggg ctccttggtg tttcatgaga aagcctggaa tgcgtacccc tactgtagaa
      421 caattgtaac gaatgaatat atgaaagatg atttcttcat taaaatcgaa acatggcaca
      481 aaccagactt gggaacatta gaaaatgtac atggtttaga tccaaacaca tggaaaactg
      541 ttgaaattgt ccatatagac attgcagata gaagtcaagt tgaaccagca gactacaaag
      601 ctgatgaaga cccagcatta ttccagtcag tcaagaccaa gagaggccct ttgggaccca
      661 actggaagaa ggagctggca aacagccctg actgtcccca gatgtgtgcc tataagctgg
      721 tgaccatcaa attcaagtgg tggggactgc aaagcaaagt agaaaacttc attcaaaagc
      781 aagaaaaacg gatatttaca aacttccatc gc
//
