LOCUS       sequence-91688-e         843 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  bacterial expression of PITP beta K61A mutant (phosphatidylinositol 
            transfer protein beta) .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 843)
  AUTHORS   Shadan S, Holic R, Carvou N, Ee P, Li M, Murray-Rust J, Cockcroft S
  TITLE     Dynamics of lipid transfer by phosphatidylinositol transfer proteins
            in cells.
  JOURNAL   Traffic. 2008 Sep;9(10):1743-56. doi: 
            10.1111/j.1600-0854.2008.00794.x. Epub 2008 Jul 9.
  PUBMED    18636990
REFERENCE   2  (bases 1 to 843)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1111/j.1600-0854.2008.00794.x"; journalName: "Traffic"; date: 
            "2008-09"; volume: "9"; issue: "10"; pages: "1743-56"
COMMENT     Sequence Label: pRSETC-PITPbeta (splice variant 1) K61A
FEATURES             Location/Qualifiers
     source          1..843
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             2..24
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             62..94
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             join(98..106,107..121)
                     /codon_start=1
                     /product="Xpress(TM) epitope tag, including an enterokinase
                     recognition and cleavage site"
                     /label=Xpress(TM) tag
                     /translation="DLYDDDDK"
ORIGIN
        1 ttttgtttaa ctttaagaag gagatataca tatgcggggt tctcatcatc atcatcatgg
       61 tatggctagc atgactggtg gacagcaaat gggtcgggat ctgtacgacg atgacgataa
      121 ggatccgatg gtgctgatca aggagttccg tgtggttttg ccatgttctg ttcaggagta
      181 tcaggttggg cagctttact ctgttgcaga agctagtaag aatgagactg gtggtggaga
      241 aggaattgaa gtcttaaaga atgaacctta tgagaaggat ggagaaaagg gacagtatac
      301 gcacgcaatt tatcacctaa agagcaaagt gcctgcattc gtgaggatga ttgctcccga
      361 gggctccttg gtgtttcatg agaaagcctg gaatgcgtac ccctactgta gaacaattgt
      421 aacgaatgaa tatatgaaag atgatttctt cattaaaatc gaaacatggc acaaaccaga
      481 cttgggaaca ttagaaaatg tacatggttt agatccaaac acatggaaaa ctgttgaaat
      541 tgtccatata gacattgcag atagaagtca agttgaacca gcagactaca aagctgatga
      601 agacccagca ttattccagt cagtcaagac caagagaggc cctttgggac ccaactggaa
      661 gaaggagctg gcaaacagcc ctgactgtcc ccagatgtgt gcctataagc tggtgaccat
      721 caaattcaag tggtggggac tgcaaagcaa agtagaaaac ttcattcaaa agcaagaaaa
      781 acggatattt acaaacttcc atcgccagct tttttgttgg attgacaagt ggatcgatct
      841 cac
//
