LOCUS       sequence-91707-e         747 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  mammalian expression of human PITP alpha .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 747)
  TITLE     Cockcroft PITP plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 747)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pcDNA3.1 myc-PITPalpha
FEATURES             Location/Qualifiers
     source          1..747
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     50..69
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        50..68
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      86..95
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             95..124
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
ORIGIN
        1 gagctctctg gctaactaga gaacccactg cttactggct tatcgaaatt aatacgactc
       61 actataggga gacccaagct ggctagccac catggaacaa aaactcatct cagaagagga
      121 tctggatccg atggtgctgc tcaaggagta tcgagtaatc ctgcctgtgt ctgtagatga
      181 gtatcaagtg gggcagctgt attctgtggc tgaggccagt aaaaatgaaa cgggtggtgg
      241 cgaaggcgtg gaggtcctgg tgaatgagcc ctacgagaag gacggtgaga aaggccagta
      301 cacacacaag atctaccacc tgcagagcaa agtacccacg tttgttcgaa tgctggcccc
      361 agagggagcc ctgaatatac acgagaaagc ctggaatgct tacccctact gcagaaccgt
      421 tattacaaat gagtacatga aagaagactt tctgattaaa attgaaacct ggcacaaacc
      481 agatcttggc acgcaggaga atgtgcataa gctggagcct gaggcgtgga aacacgtgga
      541 agccgtatat atagacattg cagatcgaag ccaagtgctc agcaaggatt acaaggcaga
      601 ggaagaccca gcaaaattta aatctatcaa aacaggccga ggacccttgg gccccaattg
      661 gaagcaagag ctggtaaacc agaaggactg cccatatatg tgtgcataca aactggtgac
      721 cgtcaagttc aagtggtggg gcctgca
//
