LOCUS       sequence-91716-e         758 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  bacterial expression of PITP beta N89F mutant .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 758)
  AUTHORS   Carvou N, Holic R, Li M, Futter C, Skippen A, Cockcroft S
  TITLE     Phosphatidylinositol- and phosphatidylcholine-transfer activity of 
            PITPbeta is essential for COPI-mediated retrograde transport from 
            the Golgi to the endoplasmic reticulum.
  JOURNAL   J Cell Sci. 2010 Apr 15;123(Pt 8):1262-73. doi: 10.1242/jcs.061986. 
            Epub 2010 Mar 23.
  PUBMED    20332109
REFERENCE   2  (bases 1 to 758)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Sci."; date: "2010-04-15"; volume: "123(Pt 8):1262-73. doi"; pages: 
            " 10.1242/jcs.061986. Epub 2010 Mar 23"
COMMENT     Sequence Label: pRSETC PITPbeta N89F
FEATURES             Location/Qualifiers
     source          1..758
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             1..23
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             43..60
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             64..96
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             join(100..108,109..123)
                     /codon_start=1
                     /product="Xpress(TM) epitope tag, including an enterokinase
                     recognition and cleavage site"
                     /label=Xpress(TM) tag
                     /translation="DLYDDDDK"
ORIGIN
        1 tttgtttaac tttaagaagg agatatacat atgcggggtt ctcatcatca tcatcatcat
       61 ggtatggcta gcatgactgg tggacagcaa atgggtcggg atctgtacga cgatgacgat
      121 aaggatccga tggtgctgat caaggagttc cgtgtggttt tgccatgttc tgttcaggag
      181 tatcaggttg ggcagcttta ctctgttgca gaagctagta agaatgagac tggtggtgga
      241 gaaggaattg aagtcttaaa gaatgaacct tatgagaagg atggagaaaa gggacagtat
      301 acgcacaaaa tttatcacct aaagagcaaa gtgcctgcat tcgtgaggat gattgctccc
      361 gagggctcct tggtgtttca tgagaaagcc tggtttgcgt acccctactg tagaacaatt
      421 gtaacgaatg aatatatgaa agatgatttc ttcattaaaa tcgaaacatg gcacaaacca
      481 gacttgggaa cattagaaaa tgtacatggt ttagatccaa acacatggaa aactgttgaa
      541 attgtccata tagacattgc agatagaagt caagttgaac cagcagacta caaagctgat
      601 gaagacccag cattattcca gtcagtcaag accaagagag gccctttggg acccaactgg
      661 aagaaggagc tggcaaacag ccctgactgt ccccagatgt gtgcctataa gctggtgacc
      721 atcaaattca agtggtgggg actgcaaaag caaaagta
//
