LOCUS       sequence-91724-e         769 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  2x35S promoting Glycing Max codon optimized Cas9 with bar plant 
            selection.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 769)
  AUTHORS   Michno JM, Wang X, Liu J, Curtin SJ, Kono TJ, Stupar RM
  TITLE     CRISPR/Cas mutagenesis of soybean and Medicago truncatula using a 
            new web-tool and a modified Cas9 enzyme.
  JOURNAL   GM Crops Food. 2015;6(4):243-52. doi: 10.1080/21645698.2015.1106063.
  PUBMED    26479970
REFERENCE   2  (bases 1 to 769)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "GM Crops 
            Food."; date: "2015"; volume: "6(4):243-52. doi"; pages: " 
            10.1080/21645698.2015.1106063"
COMMENT     Sequence Label: Cas9 MDC123
FEATURES             Location/Qualifiers
     source          1..769
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        406..750
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     694..714
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
ORIGIN
        1 ggtcaacatg gtggagcacg acacacttgt ctactccaaa aatatcaaag atacagtctc
       61 agaagaccaa agggcaattg agacttttca acaaagggta atatccggaa acctcctcgg
      121 attccattgc ccagctatct gtcactttat tgtgaagata gtggaaaagg aaggtggctc
      181 ctacaaatgc catcattgcg ataaaggaaa ggccatcgtt gaagatgcct ctgccgacag
      241 tggtcccaaa gatggacccc cacccacgag gagcatcgtg gaaaaagaag acgttccaac
      301 cacgtcttca aagcaagtgg attgatgtga taacatggtg gagcacgaca cacttgtcta
      361 ctccaaaaat atcaaagata cagtctcaga agaccaaagg gcaattgaga cttttcaaca
      421 aagggtaata tccggaaacc tcctcggatt ccattgccca gctatctgtc actttattgt
      481 gaagatagtg gaaaaggaag gtggctccta caaatgccat cattgcgata aaggaaaggc
      541 catcgttgaa gatgcctctg ccgacagtgg tcccaaagat ggacccccac ccacgaggag
      601 catcgtggaa aaagaagacg ttccaaccac gtcttcaaag caagtggatt gatgtgatat
      661 ctccactgac gtaagggatg acgcacaatc ccactatcct tcgcaagacc cttcctctat
      721 ataaggaagt tcatttcatt tggagaggac ctcgactcta gaggatccc
//
