LOCUS       sequence-91725-e         552 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  2x35S promoting Glycing Max codon optimized Cas9 with bar plant 
            selection.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 552)
  AUTHORS   Michno JM, Wang X, Liu J, Curtin SJ, Kono TJ, Stupar RM
  TITLE     CRISPR/Cas mutagenesis of soybean and Medicago truncatula using a 
            new web-tool and a modified Cas9 enzyme.
  JOURNAL   GM Crops Food. 2015;6(4):243-52. doi: 10.1080/21645698.2015.1106063.
  PUBMED    26479970
REFERENCE   2  (bases 1 to 552)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "GM Crops 
            Food."; date: "2015"; volume: "6(4):243-52. doi"; pages: " 
            10.1080/21645698.2015.1106063"
COMMENT     Sequence Label: Cas9 MDC123
FEATURES             Location/Qualifiers
     source          1..552
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             1..552
                     /codon_start=1
                     /gene="Streptomyces hygroscopicus bar"
                     /product="phosphinothricin acetyltransferase"
                     /label=BlpR
                     /note="confers resistance to bialophos or phosphinothricin"
                     /translation="MSPERRPADIRRATEADMPAVCTIVNHYIETSTVNFRTEPQEPQE
                     WTDDLVRLRERYPWLVAEVDGEVAGIAYAGPWKARNAYDWTAESTVYVSPRHQRTGLGS
                     TLYTHLLKSLEAQGFKSVVAVIGLPNDPSVRMHEALGYAPRGMLRAAGFKHGNWHDVGF
                     WQLDFSLPVPPRPVLPVTEI"
ORIGIN
        1 atgagcccag aacgacgccc ggccgacatc cgccgtgcca ccgaggcgga catgccggcg
       61 gtctgcacca tcgtcaacca ctacatcgag acaagcacgg tcaacttccg taccgagccg
      121 caggaaccgc aggagtggac ggacgacctc gtccgtctgc gggagcgcta tccctggctc
      181 gtcgccgagg tggacggcga ggtcgccggc atcgcctacg cgggcccctg gaaggcacgc
      241 aacgcctacg actggacggc cgagtcgacc gtgtacgtct ccccccgcca ccagcggacg
      301 ggactgggct ccacgctcta cacccacctg ctgaagtccc tggaggcaca gggcttcaag
      361 agcgtggtcg ctgtcatcgg gctgcccaac gacccgagcg tgcgcatgca cgaggcgctc
      421 ggatatgccc cccgcggcat gctgcgggcg gccggcttca agcacgggaa ctggcatgac
      481 gtgggtttct ggcagctgga cttcagcctg ccggtaccgc cccgtccggt cctgcccgtc
      541 accgagattt ga
//
