LOCUS       sequence-91748-e         812 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  N-terminal fusion of EGFP to Drosophila melanogaster Orco.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 812)
  AUTHORS   Benton R, Sachse S, Michnick SW, Vosshall LB
  TITLE     Atypical membrane topology and heteromeric function of Drosophila 
            odorant receptors in vivo.
  JOURNAL   PLoS Biol. 2006 Feb;4(2):e20. Epub 2006 Jan 17.
  PUBMED    16402857
REFERENCE   2  (bases 1 to 812)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS 
            Biol."; date: "2006-02"; volume: "4(2)"; pages: "e20. Epub 2006 Jan 
            17"
COMMENT     Sequence Label: RP125 pUAST EGFP:Orco
FEATURES             Location/Qualifiers
     source          1..812
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(36..47)
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     promoter        complement(58..296)
                     /label=hsp70 promoter
                     /note="Drosophila melanogaster hsp70Bb promoter"
     primer_bind     complement(90..111)
                     /label=pCasper-hs
                     /note="Drosophila Hsp70 promoter, forward primer"
     protein_bind    315..409
                     /label=5X UAS
                     /bound_moiety="GAL4"
                     /note="five tandem copies of the ""ScaI site"" 17-mer 
                     CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS) 
                     that efficiently binds yeast Gal4 (Webster et al., 1988; 
                     Pfeiffer et al., 2010)"
     misc_feature    join(471..672,673..703)
                     /label=P element 3' end
                     /note="P element 3' end"
ORIGIN
        1 ccggtgaaca gctcctcgcc cttgctcacc atgttttgca attcccaatt ccctattcag
       61 agttctcttc ttgtattcaa taattacttc ttggcagatt tcagtagttg cagttgattt
      121 acttggttgc tggttacttt taattgattc actttaactt gcactttact gcagattgtt
      181 tagcttgttc agctgcgctt gtttatttgc ttagctttcg cttagcgacg tgttcacttt
      241 gcttgtttga attgaattgt cgctccgtag acgaagcgcc tctatttata ctccggcgct
      301 cgctagagtc tccgctcgga ggacagtact ccgctcggag gacagtactc cgctcggagg
      361 acagtactcc gctcggagga cagtactccg ctcggaggac agtactccga cctgcaggca
      421 tgcaagcttg gatccggaga gctcccaacg cgttggatgc agccaagctt tgcgtactcg
      481 caaattatta aaaataaaac tttaaaaata atttcgtcta attaatatta tgagttaatt
      541 caaaccccac ggacatgcta agggttaatc aacaatcata tcgctgtctc actcagactc
      601 aatacgacac tcagaatact attcctttca ctcgcactta ttgcaagcat acgttaagtg
      661 gatgtctctt gccgacggga ccaccttatg ttatttcatc atggtctggc cattctcatc
      721 gtgagcttcc gggtgctcgc atatctggct ctaagacttc gggcccgacg caaggagtag
      781 ccgacatata tccgaaataa ctgcttgttt tt
//
