LOCUS       sequence-91751-e         894 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  N-terminal fusion of EGFP to Drosophila melanogaster Orco.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 894)
  AUTHORS   Benton R, Sachse S, Michnick SW, Vosshall LB
  TITLE     Atypical membrane topology and heteromeric function of Drosophila 
            odorant receptors in vivo.
  JOURNAL   PLoS Biol. 2006 Feb;4(2):e20. Epub 2006 Jan 17.
  PUBMED    16402857
REFERENCE   2  (bases 1 to 894)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS 
            Biol."; date: "2006-02"; volume: "4(2)"; pages: "e20. Epub 2006 Jan 
            17"
COMMENT     Sequence Label: RP125 pUAST EGFP:Orco
FEATURES             Location/Qualifiers
     source          1..894
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    62..156
                     /label=5X UAS
                     /bound_moiety="GAL4"
                     /note="five tandem copies of the ""ScaI site"" 17-mer 
                     CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS) 
                     that efficiently binds yeast Gal4 (Webster et al., 1988; 
                     Pfeiffer et al., 2010)"
     promoter        175..413
                     /label=hsp70 promoter
                     /note="Drosophila melanogaster hsp70Bb promoter"
     primer_bind     360..381
                     /label=pCasper-hs
                     /note="Drosophila Hsp70 promoter, forward primer"
     CDS             424..435
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     primer_bind     complement(484..505)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(745..764)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 aagcttggct gcatccaacg cgttgggagc tctccggatc caagcttgca tgcctgcagg
       61 tcggagtact gtcctccgag cggagtactg tcctccgagc ggagtactgt cctccgagcg
      121 gagtactgtc ctccgagcgg agtactgtcc tccgagcgga gactctagcg agcgccggag
      181 tataaataga ggcgcttcgt ctacggagcg acaattcaat tcaaacaagc aaagtgaaca
      241 cgtcgctaag cgaaagctaa gcaaataaac aagcgcagct gaacaagcta aacaatctgc
      301 agtaaagtgc aagttaaagt gaatcaatta aaagtaacca gcaaccaagt aaatcaactg
      361 caactactga aatctgccaa gaagtaatta ttgaatacaa gaagagaact ctgaataggg
      421 aattgggaat tgcaaaacat ggtgagcaag ggcgaggagc tgttcaccgg ggtggtgccc
      481 atcctggtcg agctggacgg cgacgtaaac ggccacaagt tcagcgtgtc cggcgagggc
      541 gagggcgatg ccacctacgg caagctgacc ctgaagttca tctgcaccac cggcaagctg
      601 cccgtgccct ggcccaccct cgtgaccacc ctgacctacg gcgtgcagtg cttcagccgc
      661 taccccgacc acatgaagca gcacgacttc ttcaagtccg ccatgcccga aggctacgtc
      721 caggagcgca ccatcttctt caaggacgac ggcaactaca agacccgcgc cgaggtgaag
      781 ttcgagggcg acaccctggt gaaccgcatc gagctgaagg gcatcgactt caaggaggac
      841 ggcaacatcc tggggcacaa gctggagtac aactacaaca gccacaacgt ctat
//
