LOCUS       sequence-91756-e         237 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  G10 promoting Glycing Max codon optimized Cas9 (N and C terminus 
            NLS) with bar plant selection.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 237)
  AUTHORS   Michno JM, Wang X, Liu J, Curtin SJ, Kono TJ, Stupar RM
  TITLE     CRISPR/Cas mutagenesis of soybean and Medicago truncatula using a 
            new web-tool and a modified Cas9 enzyme.
  JOURNAL   GM Crops Food. 2015;6(4):243-52. doi: 10.1080/21645698.2015.1106063.
  PUBMED    26479970
REFERENCE   2  (bases 1 to 237)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "GM Crops 
            Food."; date: "2015"; volume: "6(4):243-52. doi"; pages: " 
            10.1080/21645698.2015.1106063"
COMMENT     Sequence Label: G10 Cas9 MDC123
FEATURES             Location/Qualifiers
     source          1..237
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     175..195
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     promoter        184..230
                     /label=minimal CaMV 35S promoter
                     /note="minimal 35S promoter from cauliflower mosaic virus"
ORIGIN
        1 atagtttaaa ctgaaggcgg gaaacgacaa tctgatccaa gctcaagcta agcttgcatg
       61 cctgcaggat atcgtggatc caagcttgcc acgtgccgcc acgtgccgcc acgtgccgcc
      121 acgtgcctct agaggatcca tctccactga cgtaagggat gacgcacaat cccactatcc
      181 ttcgcaagac ccttcctcta tataaggaag ttcatttcat ttggagagga cacgctg
//
