LOCUS       sequence-91760-e         471 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Produces aminoglycoside phosphotransferase (3′)-IIa when in the 
            presence of T7 RNA Polymerase..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 471)
  AUTHORS   Schmidt CM, Shis DL, Nguyen-Huu TD, Bennett MR
  TITLE     Stable maintenance of multiple plasmids in E. coli using a single 
            selective marker.
  JOURNAL   ACS Synth Biol. 2012 Oct 19;1(10):445-50. doi: 10.1021/sb3000589. 
            Epub 2012 Sep 4.
  PUBMED    23656183
REFERENCE   2  (bases 1 to 471)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/sb3000589";
            journalName: "ACS Synth Biol"; date: "2012-10-19- 19"; volume: "1"; 
            issue: "10"; pages: "445-50"
COMMENT     Sequence Label: pCS1
FEATURES             Location/Qualifiers
     source          1..471
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(29..46)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 ttcgggcttt gttagcagcc ggatctcagt ggtggtggtg gtggtgctcg agtgcggccg
       61 caagcttgtc gacggagctc gaattcggat ccatgtgcgc ggaaccccta tttgtttatt
      121 tttctaaata cattcaaata tgtatccgct catgaattaa ttcttagaaa aactcatcga
      181 gcatcaaatg aaactgcaat ttattcatat caggattatc aataccatat ttttgaaaaa
      241 gccgtttctg taatgaagga gaaaactcac cgaggcagtt ccataggatg gcaagatcct
      301 ggtatcggtc tgcgattccg actcgtccaa catcaataca acctattaat ttcccctcgt
      361 caaaaataag gttatcaagt gagaaatcac catgagtgac gactgaatcc ggtgagaatg
      421 gcaaaagttt atgcatttct ttccagactt gttcaacagg ccagccatta c
//
