LOCUS       sequence-91771-e         551 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Serves as a shuttle vector for target oligo before insertion into 
            Cas9 destination vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 551)
  AUTHORS   Michno JM, Wang X, Liu J, Curtin SJ, Kono TJ, Stupar RM
  TITLE     CRISPR/Cas mutagenesis of soybean and Medicago truncatula using a 
            new web-tool and a modified Cas9 enzyme.
  JOURNAL   GM Crops Food. 2015;6(4):243-52. doi: 10.1080/21645698.2015.1106063.
  PUBMED    26479970
REFERENCE   2  (bases 1 to 551)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "GM Crops 
            Food."; date: "2015"; volume: "6(4):243-52. doi"; pages: " 
            10.1080/21645698.2015.1106063"
COMMENT     Sequence Label: pBlu/gRNA
FEATURES             Location/Qualifiers
     source          1..551
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_RNA        468..543
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
ORIGIN
        1 aagctttcgt tgaacaacgg aaactcgact tgccttccgc acaatacatc atttcttctt
       61 agcttttttt cttcttcttc gttcatacag tttttttttg tttatcagct tacattttct
      121 tgaaccgtag ctttcgtttt cttcttttta actttccatt cggagttttt gtatcttgtt
      181 tcatagtttg tcccaggatt agaatgatta ggcatcgaac cttcaagaat ttgattgaat
      241 aaaacatctt cattcttaag atatgaagat aatcttcaaa aggcccctgg gaatctgaaa
      301 gaagagaagc aggcccattt atatgggaaa gaacaatagt atttcttata taggcccatt
      361 taagttgaaa acaatcttca aaagtcccac atcgcttaga taagaaaacg aagctgagtt
      421 tatatacagc tagagtcgaa gtagtgattg ggtcttcgag aagacctgtt ttagagctag
      481 aaatagcaag ttaaaataag gctagtccgt tatcaacttg aaaaagtggc accgagtcgg
      541 tgcttttttt t
//
