LOCUS       sequence-91776-e        1036 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Produces one half (beta) of aminoglycoside phosphotransferase 
            (3′)-IIa when in the presence of T7 RNA Polymerase..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1036)
  AUTHORS   Schmidt CM, Shis DL, Nguyen-Huu TD, Bennett MR
  TITLE     Stable maintenance of multiple plasmids in E. coli using a single 
            selective marker.
  JOURNAL   ACS Synth Biol. 2012 Oct 19;1(10):445-50. doi: 10.1021/sb3000589. 
            Epub 2012 Sep 4.
  PUBMED    23656183
REFERENCE   2  (bases 1 to 1036)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/sb3000589";
            journalName: "ACS Synth Biol"; date: "2012-10-19- 19"; volume: "1"; 
            issue: "10"; pages: "445-50"
COMMENT     Sequence Label: pCS5
FEATURES             Location/Qualifiers
     source          1..1036
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             20..42
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     primer_bind     650..669
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     CDS             827..844
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(897..915)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      911..958
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     1001..1019
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 attcccctct agaaataatt ttgtttaact ttaagaagga gatataccat ggctagcgca
       61 cagctggaga agaaactgca ggctctggag aagaaactgg cacagctgga gtggaaaaac
      121 caggcactgg agaagaaact ggcacagggt ggaagcgggg aactgcagga cgaggcagcg
      181 cggctatcgt ggctggccac gacgggcgtt ccttgcgcag ctgtgctcga cgttgtcact
      241 gaagcgggaa gggactggct gctattgggc gaagtgccgg ggcaggatct cctgtcatct
      301 caccttgctc ctgccgagaa agtatccatc atggctgatg caatgcggcg gctgcatacg
      361 cttgatccgg ctacctgccc attcgaccac caagcgaaac atcgcatcga gcgagcacgt
      421 actcggatgg aagccggtct tgtcgatcag gatgatctgg acgaagagca tcaggggctc
      481 gcgccagccg aactgttcgc caggctcaag gcgagcatgc ccgacggcga ggatctcgtc
      541 gtgacccacg gcgatgcctg cttgccgaat atcatggagg aaaatggccg cttttctgga
      601 ttcatcgact gtggccggct gggtgtggcg gaccgctatc aggacatagc gttggctacc
      661 cgtgatattg ccgaagagct tggcggcgaa tgggctgacc gcttcctcgt gctttacggt
      721 atcgccgctc ccgattcgca gcgcatcgcc ttctatcgcc ttcttgacga gttcttctaa
      781 ggatccgaat tcgagctccg tcgacaagct tgcggccgca ctcgagcacc accaccacca
      841 ccactgagat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc
      901 tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgga
      961 cgtctaagaa accattatta tcatgacatt aacctataaa aataggcgta tcacgaggcc
     1021 ctttcgtctt caagaa
//
