LOCUS       sequence-91777-e         809 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Nucleus/Histones, Excitation: 487, Emission: 509.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 809)
  TITLE     Davidson mEmerald
REFERENCE   2  (bases 1 to 809)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mEmerald-H2B-N-22
FEATURES             Location/Qualifiers
     source          1..809
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      10..19
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    27..79
                     /label=MCS
                     /note="multiple cloning site"
     CDS             complement(80..457)
                     /codon_start=1
                     /gene="HIST1H2BJ"
                     /product="human histone H2B"
                     /label=H2B
                     /translation="MPEPAKSAPAPKKGSKKAVTKAQKKGGKKRKRSRKESYSIYVYKV
                     LKQVHPDTGISSKAMGIMNSFVNDIFERIAGEASRLAHYNKRSTITSREIQTAVRLLLP
                     GELAKHAVSEGTKAITKYTSAK"
     promoter        complement(492..695)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(521..541)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
ORIGIN
        1 ccttgctcac catggtggcg accggtggat cccgggcccg cggtaccgtc gactgcagaa
       61 ttcgaagctt gagctcgagc ttagcgctgg tgtacttggt gatggcctta gtaccctcgg
      121 acacggcgtg cttggccaac tccccaggca gcagcaggcg cacggccgtc tggatctccc
      181 tggaggtgat ggtcgagcgc ttgttgtaat gcgccaggcg ggaagcctca cctgcgatgc
      241 gctcgaaaat gtcgttcaca aacgaattca tgatgcccat ggccttggac gaaatgccgg
      301 tgtcagggtg gacctgcttc agaaccttgt acacatagat ggaatagctc tccttgcggc
      361 tgcgcttgcg cttcttgccg cctttcttct gcgccttagt caccgccttc ttggagccct
      421 ttttcggggc gggagcagac ttcgctggct ctggcatggt ggcgctagcg gatctgacgg
      481 ttcactaaac cagctctgct tatatagacc tcccaccgta cacgcctacc gcccatttgc
      541 gtcaatgggg cggagttgtt acgacatttt ggaaagtccc gttgattttg gtgccaaaac
      601 aaactcccat tgacgtcaat ggggtggaga cttggaaatc cccgtgagtc aaaccgctat
      661 ccacgcccat tgatgtactg ccaaaaccgc atcaccatgg taatagcgat gactaatacg
      721 tagatgtact gccaagtagg aaagtcccat aaggtcatgt actgggcata atgccaggcg
      781 ggccatttac cgtcattgac gtcaatagg
//
