LOCUS       sequence-91778-e        1029 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Produces one half (beta) of aminoglycoside phosphotransferase 
            (3′)-IIa when in the presence of T7 RNA Polymerase..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1029)
  AUTHORS   Schmidt CM, Shis DL, Nguyen-Huu TD, Bennett MR
  TITLE     Stable maintenance of multiple plasmids in E. coli using a single 
            selective marker.
  JOURNAL   ACS Synth Biol. 2012 Oct 19;1(10):445-50. doi: 10.1021/sb3000589. 
            Epub 2012 Sep 4.
  PUBMED    23656183
REFERENCE   2  (bases 1 to 1029)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/sb3000589";
            journalName: "ACS Synth Biol"; date: "2012-10-19- 19"; volume: "1"; 
            issue: "10"; pages: "445-50"
COMMENT     Sequence Label: pCS5
FEATURES             Location/Qualifiers
     source          1..1029
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(31..48)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(206..225)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     RBS             833..855
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    870..894
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(894..913)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(895..913)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 ctttcgggct ttgttagcag ccggatctca gtggtggtgg tggtggtgct cgagtgcggc
       61 cgcaagcttg tcgacggagc tcgaattcgg atccttagaa gaactcgtca agaaggcgat
      121 agaaggcgat gcgctgcgaa tcgggagcgg cgataccgta aagcacgagg aagcggtcag
      181 cccattcgcc gccaagctct tcggcaatat cacgggtagc caacgctatg tcctgatagc
      241 ggtccgccac acccagccgg ccacagtcga tgaatccaga aaagcggcca ttttcctcca
      301 tgatattcgg caagcaggca tcgccgtggg tcacgacgag atcctcgccg tcgggcatgc
      361 tcgccttgag cctggcgaac agttcggctg gcgcgagccc ctgatgctct tcgtccagat
      421 catcctgatc gacaagaccg gcttccatcc gagtacgtgc tcgctcgatg cgatgtttcg
      481 cttggtggtc gaatgggcag gtagccggat caagcgtatg cagccgccgc attgcatcag
      541 ccatgatgga tactttctcg gcaggagcaa ggtgagatga caggagatcc tgccccggca
      601 cttcgcccaa tagcagccag tcccttcccg cttcagtgac aacgtcgagc acagctgcgc
      661 aaggaacgcc cgtcgtggcc agccacgata gccgcgctgc ctcgtcctgc agttccccgc
      721 ttccaccctg tgccagtttc ttctccagtg cctggttttt ccactccagc tgtgccagtt
      781 tcttctccag agcctgcagt ttcttctcca gctgtgcgct agccatggta tatctccttc
      841 ttaaagttaa acaaaattat ttctagaggg gaattgttat ccgctcacaa ttcccctata
      901 gtgagtcgta ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc
      961 ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg
     1021 cggtatcat
//
