LOCUS       sequence-91780-e         972 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Inducible expression of E gene which produces a lysis protein that 
            induces autolysis..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 972)
  AUTHORS   Tanouchi Y, Pai A, Buchler NE, You L
  TITLE     Programming stress-induced altruistic death in engineered bacteria.
  JOURNAL   Mol Syst Biol. 2012;8:626. doi: 10.1038/msb.2012.57.
  PUBMED    23169002
REFERENCE   2  (bases 1 to 972)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Syst 
            Biol."; date: "2012"; volume: "8:626. doi"; pages: " 
            10.1038/msb.2012.57"
COMMENT     Sequence Label: pCSaE500
FEATURES             Location/Qualifiers
     source          1..972
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     113..132
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        113..131
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    132..156
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
ORIGIN
        1 ctgattgact ctcttccggg cgctatcatg ccataccgcg aaaggttttg cgccattcga
       61 tggtgtccgg gatctcgacg ctctccctta tgcgactcct gcattaggaa attaatacga
      121 ctcactatag gggaattgtg agcggataac aattcccctg tagaaataat tttgtttaac
      181 tttaataagg agatatacca tggctcgagg tattctgacc atctaagtac atgcgttaat
      241 tatcaggccc gctattggcg ggcctttttt atttgtgcag caccccggtc aaccaacggg
      301 aaaattcacg catcgcgggc gtctccgggc gagattgcaa acgcgttatc cagtagcttc
      361 ccaaatcaat ctgcgttaaa aacggctgaa cgatacgttc actgctgagt aaatgcgtga
      421 acattctgac tggcgcaatt gccacaccca ttcccgcctg tgcagcttcc aacatggtga
      481 ctgacgaatc aaacaccatc acattgtgcg tcggtgacgg aggcgcctct ccggccgcct
      541 gcatccaaag cgcccattca tcccgccgat acgatcgtaa taacggaaat ttcaggatat
      601 ccgcaggcgt ttgaatctgc gaagccaatg ttggcgaaca cagtggagac atcagcgcgc
      661 tacataaata ttgcgcatcg gtatcgtgcc aggctccccc accgtagcga atggtataat
      721 ccagcccttc ggcggcggga tccacgcgat tgttatgggt agaaatatgc aaatcaatat
      781 gtgggtaact gcgcttaaaa tcgctcaaca gcgggaaaag acagccgata gcaaaggttc
      841 ccactacgcc aattttaagt ttttcctggg tctgtttagt ggcaaaacga tccaacatcc
      901 ccgccatacg atcgaaggag tcattaagta caggtaacag actttcccct tcagtcgtca
      961 acattaatcc ac
//
