LOCUS       sequence-91782-e         850 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  tol2 transgenesis for biotin based TRAP - BirA ligase.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 850)
  AUTHORS   Housley MP, Reischauer S, Dieu M, Raes M, Stainier DY, Vanhollebeke 
            B
  TITLE     Translational profiling through biotinylation of tagged ribosomes in
            zebrafish.
  JOURNAL   Development. 2014 Oct;141(20):3988-93. doi: 10.1242/dev.111849. Epub
            2014 Sep 17.
  PUBMED    25231762
REFERENCE   2  (bases 1 to 850)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1242/dev.111849"; journalName: "Development"; date: "2014-10"; 
            volume: "141"; issue: "20"; pages: "3988-93"
COMMENT     Sequence Label: pT2KXIGdeltaIn-MCS-birA-EGFP
FEATURES             Location/Qualifiers
     source          1..850
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    join(77..88,89..251)
                     /label=Tol2 right end
                     /note="right end of the Tol2 transposon from the medaka 
                     fish Oryzias latipes (Kawakami, 2007)"
ORIGIN
        1 aagacgtgaa tatctgttca gacacccata tccactctgt tccacacagg tcagaggttt
       61 gtccaggagt tcttgacaga ggtgtaaaaa gtactcaaaa attttactca agtgaaagta
      121 caagtactta gggaaaattt tactcaatta aaagtaaaag tatctggcta gaatcttact
      181 tgagtaaaag taaaaaagta ctccattaaa attgtacttg agtattaagg aagtaaaagt
      241 aaaagcaaga aagaaaacta gagattcttg tttaagcttt taatctcaaa aaacattaaa
      301 tgaaatgcat acaaggtttt atcctgcttt agaactgttt gtatttaatt atcaaactat
      361 aagacagaca atctaatgcc agtacacgct actcaaagtt gtaaaacctc agatttaact
      421 tcagtagaag ctgattctca aaattgttag tgtcaagcct agctcttttg gggctgaaaa
      481 gcaatcctgc agtgctgaaa agcctctcac aggcagccga tgcgggaaga ggtgtattag
      541 tcttgataga gaggctgcaa atagcaggaa acgtgagcag agactccctg gtgtctgaaa
      601 cacaggccag atgggccctc gagttaatta aaggcctggc cggccgaatt cggatccgcc
      661 accatgaagg ataacaccgt gccactgaaa ttgattgccc tgttagcgaa cggtgaattt
      721 cactctggcg agcagttggg tgaaacgctg ggaatgagcc gggcggctat taataaacac
      781 attcagacac tgcgtgactg gggcgttgat gtctttaccg ttccgggtaa aggatacagc
      841 ctgcctgagc
//
