LOCUS       sequence-91784-e         966 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  tol2 transgenesis for biotin based TRAP - BirA ligase.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 966)
  AUTHORS   Housley MP, Reischauer S, Dieu M, Raes M, Stainier DY, Vanhollebeke 
            B
  TITLE     Translational profiling through biotinylation of tagged ribosomes in
            zebrafish.
  JOURNAL   Development. 2014 Oct;141(20):3988-93. doi: 10.1242/dev.111849. Epub
            2014 Sep 17.
  PUBMED    25231762
REFERENCE   2  (bases 1 to 966)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1242/dev.111849"; journalName: "Development"; date: "2014-10"; 
            volume: "141"; issue: "20"; pages: "3988-93"
COMMENT     Sequence Label: pT2KXIGdeltaIn-MCS-birA-EGFP
FEATURES             Location/Qualifiers
     source          1..966
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      29..38
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 ccggtgaaca gctcctcgcc cttgctcacc atggtggcaa gctttttttc tgcactacgc
       61 agggatattt caccgcccat ccagggtttt attattccat cctgctcaag taataaagcc
      121 ccctgtttgt ctattccgcg tgaaatgcca aatatttctt tatcaccaat gataagtttc
      181 actgggcgat taataaaatt atccagcttt tcccagcgcg acagataagg tgccaatcct
      241 tcttgttcga agagttccaa cgcagcacgt aattcacgta ttagcatggc cgccaacgta
      301 ttacgatcga gattgatccc cgcttcctgc agcgtgatcc acccctgatt aacgacactc
      361 tcttcaacac ggcgcattgc catgttgatc ccggctccaa tgactatttg cgccgcatcg
      421 ccagttttgc cagtcagctc caccagaatg cctgccagct tgcgatcctg cagatagagg
      481 tcattaggcc atttaacacg aactttatct gcacccagct tgcgtaatac ttccgccatc
      541 acgataccga taaccagact taaaccaatc gccgccgccg ggccttgttc cagacgccag
      601 aacatcgaca aatataagtt tgcgccaaaa ggcgaaaacc atttccgacc ccggcgacca
      661 cggccagcct gctggtattc tgcaatgcaa gcatcgcccg atttaagctc tccgatacga
      721 tcaagaaggt actgattcgt ggagtcaatc acaggcagca cggctacact accgccatcc
      781 agctgaccca atatctgttt agcattaagt aactggatag gctcaggcag gctgtatcct
      841 ttacccggaa cggtaaagac atcaacgccc cagtcacgca gtgtctgaat gtgtttatta
      901 atagccgccc ggctcattcc cagcgtttca cccaactgct cgccagagtg aaattcaccg
      961 ttcgct
//
