LOCUS       sequence-91787-e        1032 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  tol2 transgenesis for biotin based TRAP - BirA ligase.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1032)
  AUTHORS   Housley MP, Reischauer S, Dieu M, Raes M, Stainier DY, Vanhollebeke 
            B
  TITLE     Translational profiling through biotinylation of tagged ribosomes in
            zebrafish.
  JOURNAL   Development. 2014 Oct;141(20):3988-93. doi: 10.1242/dev.111849. Epub
            2014 Sep 17.
  PUBMED    25231762
REFERENCE   2  (bases 1 to 1032)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1242/dev.111849"; journalName: "Development"; date: "2014-10"; 
            volume: "141"; issue: "20"; pages: "3988-93"
COMMENT     Sequence Label: pT2KXIGdeltaIn-MCS-birA-EGFP
FEATURES             Location/Qualifiers
     source          1..1032
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     23..43
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        25..43
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     80..96
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     80..96
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     misc_feature    join(130..141,142..329)
                     /label=Tol2 left end
                     /note="left end of the Tol2 transposon from the medaka fish
                     Oryzias latipes (Kawakami, 2007)"
     polyA_signal    768..902
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(805..824)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     859..878
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(992..1013)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 tgaccatgat tacgccaagc gcgcaattaa ccctcactaa agggaacaaa agctggagct
       61 ccaccgcggt ggcggccgct ctagaactag tggatctgct gggcttgctg aaggtagggg
      121 gtcaagaacc agaggtgtaa agtacttgag taattttact tgattactgt acttaagtat
      181 tatttttggg gatttttact ttacttgagt acaattaaaa atcaatactt ttacttttac
      241 ttaattacat ttttttagaa aaaaaagtac tttttactcc ttacaatttt atttacagtc
      301 aaaaagtact tattttttgg agatcacttc attctatttt cccttgctat taccaaacca
      361 attgaattgc gctgatgccc agtttaattt aaatgttatt tattctgcct atgaaaatcg
      421 ttttcacatt atatgaaatt ggtcagacat gttcattggt cctttggaag tgacgtcatg
      481 tcacatctat taccacaatg cacagcacct tgacctggaa attagggaaa ttataacagt
      541 caatcagtgg aagaaaatgg aggaagtatg tgattcatca gcagctgcga gcagcacagt
      601 ccaaaatcag ccacaggatc aagagcaccc gtggccgtat cttcgcagat ctgatctaga
      661 ggatcataat cagccatacc acatttgtag aggttttact tgctttaaaa aacctcccac
      721 acctccccct gaacctgaaa cataaaatga atgcaattgt tgttgttaac ttgtttattg
      781 cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt
      841 tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctgga
      901 tcatcatcga tgaattaatt cgagctccac cgcggtggcg gccgctttac ttgtacagct
      961 cgtccatgcc gagagtgatc ccggcggcgg tcacgaactc cagcaggacc atgtgatcgc
     1021 gcttctcgtt gg
//
