LOCUS       sequence-91793-e         779 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 779)
  TITLE     Davidson mEmerald
REFERENCE   2  (bases 1 to 779)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mEmerald-Hec1-N-12
FEATURES             Location/Qualifiers
     source          1..779
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    140..261
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(177..196)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     231..250
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(268..723)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(405..426)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     617..636
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 gacgagctgt acaagtaagc ggccgcgact ctagatcata atcagccata ccacatttgt
       61 agaggtttta cttgctttaa aaaacctccc acacctcccc ctgaacctga aacataaaat
      121 gaatgcaatt gttgttgtta acttgtttat tgcagcttat aatggttaca aataaagcaa
      181 tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc
      241 caaactcatc aatgtatctt aaggcgtaaa ttgtaagcgt taatattttg ttaaaattcg
      301 cgttaaattt ttgttaaatc agctcatttt ttaaccaata ggccgaaatc ggcaaaatcc
      361 cttataaatc aaaagaatag accgagatag ggttgagtgt tgttccagtt tggaacaaga
      421 gtccactatt aaagaacgtg gactccaacg tcaaagggcg aaaaaccgtc tatcagggcg
      481 atggcccact acgtgaacca tcaccctaat caagtttttt ggggtcgagg tgccgtaaag
      541 cactaaatcg gaaccctaaa gggagccccc gatttagagc ttgacgggga aagccggcga
      601 acgtggcgag aaaggaaggg aagaaagcga aaggagcggg cgctagggcg ctggcaagtg
      661 tagcggtcac gctgcgcgta accaccacac ccgccgcgct taatgcgccg ctacagggcg
      721 cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaa
//
