LOCUS       sequence-91804-e         856 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Actin, Excitation: 503 / 569, Emission: 514 / 581.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 856)
  AUTHORS   Davidson Lab
  TITLE     Michael Davidson Empty Backbones
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 856)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: mGeos2-VESS-Lifeact-7
FEATURES             Location/Qualifiers
     source          1..856
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             66..116
                     /codon_start=1
                     /product="peptide that binds filamentous actin (Riedl et 
                     al., 2008)"
                     /label=Lifeact
                     /note="first 17 amino acids of budding yeast Abp140"
                     /translation="MGVADLIKKFESISKEE"
     CDS             126..806
                     /codon_start=1
                     /product="truly monomeric reversibly photoswitchable 
                     fluorescent protein, suitable for nonlinear structured 
                     illumination microscopy (Zhang et al., 2016)"
                     /label=Skylan-NS
                     /note="mEos3.1 derivative that eliminates the green-to-red 
                     conversion"
                     /translation="MSAIKPDMKIKLRMEGNVNGHHFVIDGDGTGKPFEGKQSMDLEVK
                     EGGPLPFAFDILTTAFSYGNRVFAKYPDNIQDYFKQSFPKGYSWERSLTFEDGGICNAR
                     NDITMEGDTFYNKVRFYGTNFPANGPVMQKKTLKWEPSTEKMYVRDGVLTGDVEMALLL
                     EGNAHYRCDSRTTYKAKEKGVKLPGAHFVDHCIEILSHDKDYNKVKLYEHAVAHSGLPD
                     NARR"
     CDS             126..806
                     /codon_start=1
                     /product="improved monomeric variant of green-to-red 
                     photoswitchable fluorescent protein EosFP (Zhang et al., 
                     2012)"
                     /label=mEos3.1
                     /translation="MSAIKPDMKIKLRMEGNVNGHHFVIDGDGTGKPFEGKQSMDLEVK
                     EGGPLPFAFDILTTAFSYGNRVFAKYPDNIQDYFKQSFPKGYSWERSLTFEDGGICNAR
                     NDITMEGDTFYNKVRFYGTNFPANGPVMQKKTLKWEPSTEKMYVRDGVLTGDVEMALLL
                     EGNAHYRCDSRTTYKAKEKGVKLPGAHFVDHCIEILSHDKDYNKVKLYEHAVAHSGLPD
                     NARR"
ORIGIN
        1 gtgaaccgtc agatccgcta gcgctaccgg actcagatct cgagctcaag cttcgaattc
       61 ccaccatggg tgtcgcagat ttgatcaaga aattcgaaag catctcaaag gaagaacggg
      121 atccaatgag tgcgattaag ccagacatga agatcaaact ccgtatggaa ggcaacgtaa
      181 acgggcacca ctttgtgatc gacggagatg gtacaggcaa gccttttgag ggaaaacaga
      241 gtatggatct tgaagtcaaa gagggcggac ctctgccttt tgcctttgat atcctgacca
      301 ctgcattctc ctacggcaac agggtattcg ccaaatatcc agacaacata caagactatt
      361 ttaagcagtc gtttcctaag gggtattcgt gggaacgaag cttgactttc gaagacgggg
      421 gcatttgcaa cgccagaaac gacataacaa tggaagggga cactttctat aataaagttc
      481 gattttatgg taccaacttt cccgccaatg gtccagttat gcagaagaag acgctgaaat
      541 gggagccctc cactgagaaa atgtatgtgc gtgatggagt gctgacgggt gatgtagaga
      601 tggctttgtt gcttgaagga aatgcccatt accgatgtga ctccagaact acttacaaag
      661 ctaaggagaa gggtgtcaag ttaccaggcg cccactttgt ggaccactgc attgagattt
      721 taagccatga caaagattac aacaaggtta agctgtatga gcatgctgtt gctcattctg
      781 gattgcctga caatgccaga cgataagcgg ccgcgactct agatcataat cagccatacc
      841 acatttgtag aggttt
//
