LOCUS       sequence-91808-e         861 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: C1 Cloning Vector, Excitation: 503 / 569, Emission: 
            514 / 581.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 861)
  AUTHORS   Davidson Lab
  TITLE     Michael Davidson Empty Backbones
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 861)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: mGeos2-VEL-C1
FEATURES             Location/Qualifiers
     source          1..861
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             35..712
                     /codon_start=1
                     /product="truly monomeric reversibly photoswitchable 
                     fluorescent protein, suitable for nonlinear structured 
                     illumination microscopy (Zhang et al., 2016)"
                     /label=Skylan-NS
                     /note="mEos3.1 derivative that eliminates the green-to-red 
                     conversion"
                     /translation="MSAIKPDMKIKLRMEGNVNGHHFVIDGDGTGKPFEGKQSMDLEVK
                     EGGPLPFAFDILTTAFLYGNRVFAKYPDNIQDYFKQSFPKGYSWERSLTFEDGGICNAR
                     NDITMEGDTFYNKVRFYGTNFPANGPVMQKKTLKWEPSTEKMYVRDGVLTGDVEMALLL
                     EGNAHYRCDFRTTYKAKEKGVKLPGAHFVDHCIEILSHDKDYNKVKLYEHAVAHSGLPD
                     NARR"
     misc_feature    713..778
                     /label=MCS
                     /note="multiple cloning site"
ORIGIN
        1 accgtcagat ccgctagcgc taccggtcgc caccatgagt gcgattaagc cagacatgaa
       61 gatcaaactc cgtatggaag gcaacgtaaa cgggcaccac tttgtgatcg acggagatgg
      121 tacaggcaag ccttttgagg gaaaacagag tatggatctt gaagtcaaag agggcggacc
      181 tctgcctttt gcctttgata tcctgaccac tgcattcctg tacggcaaca gggtattcgc
      241 caaatatcca gacaacatac aagactattt taagcagtcg tttcctaagg ggtattcgtg
      301 ggaacgaagc ttgactttcg aagacggggg catttgcaac gccagaaacg acataacaat
      361 ggaaggggac actttctata ataaagttcg attttatggt accaactttc ccgccaatgg
      421 tccagttatg cagaagaaga cgctgaaatg ggagccctcc actgagaaaa tgtatgtgcg
      481 tgatggagtg ctgacgggtg atgtagagat ggctttgttg cttgaaggaa atgcccatta
      541 ccgatgtgac ttcagaacta cttacaaagc taaggagaag ggtgtcaagt taccaggcgc
      601 ccactttgtg gaccactgca ttgagatttt aagccatgac aaagattaca acaaggttaa
      661 gctgtatgag catgctgttg ctcattctgg attgcctgac aatgccagac gatccggact
      721 cagatctcga gctcaagctt cgaattctgc agtcgacggt accgcgggcc cgggatccac
      781 cggatctaga taactgatca taatcagcca taccacattt gtagaggttt tacttgcttt
      841 aaaaaacctc ccacacctcc c
//
