LOCUS       sequence-91809-e        1074 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses LexA-ER-VP16 in yeast cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1074)
  AUTHORS   Ottoz DS, Rudolf F, Stelling J
  TITLE     Inducible, tightly regulated and growth condition-independent 
            transcription factor in Saccharomyces cerevisiae.
  JOURNAL   Nucleic Acids Res. 2014 Jul 17. pii: gku616.
  PUBMED    25034689
REFERENCE   2  (bases 1 to 1074)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2014 Jul 17. pii: gku616."
COMMENT     Sequence Label: FRP467-PACT1(-1-520)-LexA-ER-haVP16
FEATURES             Location/Qualifiers
     source          1..1074
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             623..874
                     /codon_start=1
                     /gene="lexA (truncated)"
                     /product="DNA-binding domain of E. coli LexA"
                     /label=LexA BD
                     /note="binds the 16-bp palindromic sequence 
                     5'-CTGTATATATATACAG-3'"
                     /translation="MKALTARQQEVFDLIRDHISQTGMPPTRAEIAQRLGFRSPNAAEE
                     HLKALARKGVIEIVSGASRGIRLLQEEEEGLPLVGRVAA"
ORIGIN
        1 gcggccgcct atagggcgaa ttggagctcc accgcggtgg cggccgctct agagaagcgg
       61 gtaagctgcc acagcaatta atgcacaaca tttaacctac attcttcctt atcggatcct
      121 caaaaccctt aaaaacatat gcctcaccct aacatatttt ccaattaacc ctcaatattt
      181 ctctgtcacc cggcctctat tttccatttt cttctttacc cgccacgcgt ttttttcttt
      241 caaatttttt tcttccttct tctttttctt ccacgtcctc ttgcataaat aaataaaccg
      301 ttttgaaacc aaactcgcct ctctctctcc tttttgaaat atttttgggt ttgtttgatc
      361 ctttccttcc caatctctct tgtttaatat atattcattt atatcacgct ctctttttat
      421 cttccttttt ttcctctctc ttgtattctt ccttcccctt tctactcaaa ccaagaagaa
      481 aaagaaaagg tcaatctttg ttaaagaata ggatcttcta ctacatcagc ttttagattt
      541 ttcacgctta ctgctttttt cttcccaaga tcgaaaattt actgaattaa caactagtgg
      601 atcccccggg ctgcaggaat tcatgaaagc gttaacggcc aggcaacaag aggtgtttga
      661 tctcatccgt gatcacatca gccagacagg tatgccgccg acgcgtgcgg aaatcgcgca
      721 gcgtttgggg ttccgttccc caaacgcggc tgaagaacat ctgaaggcgc tggcacgcaa
      781 aggcgttatt gaaattgttt ccggcgcatc acgcgggatt cgtctgttgc aggaagagga
      841 agaagggttg ccgctggtag gtcgtgtggc tgccggtgaa ccacttctgg cgcaacagca
      901 tattgaaggt cattatcagg tcgatccttc cttattcaag ccgaatgctg atttcctgct
      961 gcgcgtcagc gggatgtcga tgaaagacat cggcattatg gatggtgact tgctggcagt
     1021 gcataaaact caggatgtac gtaacggtca ggtcgttgtc gcacgtattg atga
//
