LOCUS       sequence-91811-e         898 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses LexA-ER-VP16 in yeast cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 898)
  AUTHORS   Ottoz DS, Rudolf F, Stelling J
  TITLE     Inducible, tightly regulated and growth condition-independent 
            transcription factor in Saccharomyces cerevisiae.
  JOURNAL   Nucleic Acids Res. 2014 Jul 17. pii: gku616.
  PUBMED    25034689
REFERENCE   2  (bases 1 to 898)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2014 Jul 17. pii: gku616."
COMMENT     Sequence Label: FRP467-PACT1(-1-520)-LexA-ER-haVP16
FEATURES             Location/Qualifiers
     source          1..898
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     25..41
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     26..42
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             complement(69..302)
                     /codon_start=1
                     /gene="UL48"
                     /product="transcriptional activation domain of herpes 
                     simplex virus protein VP16 (Triezenberg et al., 1988; 
                     Cousens et al., 1989)"
                     /label=VP16 AD
                     /translation="APPTDVSLGDELHLDGEDVAMAHADALDDFDLDMLGDGDSPGPGF
                     TPHDSAPYGALDMADFEFEQMFTDALGIDEYGG"
ORIGIN
        1 cgaagctggg taccgggccc cccctcgagg tcgacggtat cgataagctt taaacgcgtt
       61 taaagcttcc caccgtactc gtcaattcca agggcatcgg taaacatctg ctcaaactcg
      121 aagtcggcca tatccagagc gccgtagggg gcggagtcgt ggggggtaaa tcccggaccc
      181 ggggaatccc cgtcccccaa catgtccaga tcgaaatcgt ctagcgcgtc ggcatgcgcc
      241 atcgccacgt cctcgccgtc taagtgcaat tcgtccccca ggctgacatc ggtcgggggg
      301 gccgtcgaca gtctgcgcgt gtgtcccgcg gggagaaagg acaggcgcgg agccgccagc
      361 cccgcctctt cgggggcgtc gtcgtccggg agatcgagca ggccctcgat ggtagacccg
      421 taattgtttt tcgtacgcgc gcccgcggtg ctgttatcgt tgcccggcag atcctgcatg
      481 atatcgactg tggcagggaa accctctgcc tcccccgtga tgtaatactt ttgcaaggaa
      541 tgcgatgaag tagagcccgc agtggccaag tggctttggt ccgtctcctc cacggatgcc
      601 cctccacggc tagtgggcgc atgtaggcgg tgggcgtcca gcatctccag cagcaggtca
      661 tagaggggca ccacgttctt gcacttcatg ctgtacagat gctccatgcc tttgttactc
      721 atgtgcctga tgtgggagag gatgaggagg agctgggcca gccgctggtg ctgctgctgc
      781 agggtcaggc ctgccttggc catcaggtgg atcaaagtgt ctgtgatctt gtccaggact
      841 cggtggatat ggtccttctc ttccagagac ttcagggtgc tggacagaaa tgtgtaca
//
