LOCUS       sequence-91814-e         847 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: C1 Cloning Vector, Excitation: 503 / 569, Emission: 
            514 / 581.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 847)
  AUTHORS   Davidson Lab
  TITLE     Michael Davidson Empty Backbones
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 847)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: mGeos2-VESS-C1
FEATURES             Location/Qualifiers
     source          1..847
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             53..730
                     /codon_start=1
                     /product="truly monomeric reversibly photoswitchable 
                     fluorescent protein, suitable for nonlinear structured 
                     illumination microscopy (Zhang et al., 2016)"
                     /label=Skylan-NS
                     /note="mEos3.1 derivative that eliminates the green-to-red 
                     conversion"
                     /translation="MSAIKPDMKIKLRMEGNVNGHHFVIDGDGTGKPFEGKQSMDLEVK
                     EGGPLPFAFDILTTAFSYGNRVFAKYPDNIQDYFKQSFPKGYSWERSLTFEDGGICNAR
                     NDITMEGDTFYNKVRFYGTNFPANGPVMQKKTLKWEPSTEKMYVRDGVLTGDVEMALLL
                     EGNAHYRCDSRTTYKAKEKGVKLPGAHFVDHCIEILSHDKDYNKVKLYEHAVAHSGLPD
                     NARR"
     CDS             53..730
                     /codon_start=1
                     /product="improved monomeric variant of green-to-red 
                     photoswitchable fluorescent protein EosFP (Zhang et al., 
                     2012)"
                     /label=mEos3.1
                     /translation="MSAIKPDMKIKLRMEGNVNGHHFVIDGDGTGKPFEGKQSMDLEVK
                     EGGPLPFAFDILTTAFSYGNRVFAKYPDNIQDYFKQSFPKGYSWERSLTFEDGGICNAR
                     NDITMEGDTFYNKVRFYGTNFPANGPVMQKKTLKWEPSTEKMYVRDGVLTGDVEMALLL
                     EGNAHYRCDSRTTYKAKEKGVKLPGAHFVDHCIEILSHDKDYNKVKLYEHAVAHSGLPD
                     NARR"
     misc_feature    731..796
                     /label=MCS
                     /note="multiple cloning site"
ORIGIN
        1 gcagagctgg tttagtgaac cgtcagatcc gctagcgcta ccggtcgcca ccatgagtgc
       61 gattaagcca gacatgaaga tcaaactccg tatggaaggc aacgtaaacg ggcaccactt
      121 tgtgatcgac ggagatggta caggcaagcc ttttgaggga aaacagagta tggatcttga
      181 agtcaaagag ggcggacctc tgccttttgc ctttgatatc ctgaccactg cattctccta
      241 cggcaacagg gtattcgcca aatatccaga caacatacaa gactatttta agcagtcgtt
      301 tcctaagggg tattcgtggg aacgaagctt gactttcgaa gacgggggca tttgcaacgc
      361 cagaaacgac ataacaatgg aaggggacac tttctataat aaagttcgat tttatggtac
      421 caactttccc gccaatggtc cagttatgca gaagaagacg ctgaaatggg agccctccac
      481 tgagaaaatg tatgtgcgtg atggagtgct gacgggtgat gtagagatgg ctttgttgct
      541 tgaaggaaat gcccattacc gatgtgactc cagaactact tacaaagcta aggagaaggg
      601 tgtcaagtta ccaggcgccc actttgtgga ccactgcatt gagattttaa gccatgacaa
      661 agattacaac aaggttaagc tgtatgagca tgctgttgct cattctggat tgcctgacaa
      721 tgccagacga tccggactca gatctcgagc tcaagcttcg aattctgcag tcgacggtac
      781 cgcgggcccg ggatccaccg gatctagata actgatcata atcagccata ccacatttgt
      841 agaggtt
//
