LOCUS       sequence-91815-e         857 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: N1 Cloning Vector, Excitation: 399, Emission: 456.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 857)
  AUTHORS   Subach OM, Cranfill PJ, Davidson MW, Verkhusha VV
  TITLE     An enhanced monomeric blue fluorescent protein with the high 
            chemical stability of the chromophore.
  JOURNAL   PLoS One. 2011;6(12):e28674. Epub 2011 Dec 8.
  PUBMED    22174863
REFERENCE   2  (bases 1 to 857)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2011"; volume: "6(12)"; pages: "e28674. Epub 2011 Dec 8"
COMMENT     Sequence Label: mTagBFP2-N1
FEATURES             Location/Qualifiers
     source          1..857
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    17..97
                     /label=MCS
                     /note="multiple cloning site"
     regulatory      99..108
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             105..815
                     /codon_start=1
                     /product="enhanced monomeric blue fluorescent protein 
                     (Subach et al., 2011)"
                     /label=mTagBFP2
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTM
                     RIKVVEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGG
                     VLTATQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRND
                     MALKLVGGSHLIANAKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAV
                     ARYCDLPSKLGHKLN"
ORIGIN
        1 gtgaaccgtc agatccgcta gcgctaccgg actcagatct cgagctcaag cttcgaattc
       61 tgcagtcgac ggtaccgcgg gcccgggatc caccggtcgc caccatggtg tctaagggcg
      121 aagagctgat taaggagaac atgcacatga agctgtacat ggagggcacc gtggacaacc
      181 atcacttcaa gtgcacatcc gagggcgaag gcaagcccta cgagggcacc cagaccatga
      241 gaatcaaggt ggtcgagggc ggccctctcc ccttcgcctt cgacatcctg gctactagct
      301 tcctctacgg cagcaagacc ttcatcaacc acacccaggg catccccgac ttcttcaagc
      361 agtccttccc tgagggcttc acatgggaga gagtcaccac atacgaagac gggggcgtgc
      421 tgaccgctac ccaggacacc agcctccagg acggctgcct catctacaac gtcaagatca
      481 gaggggtgaa cttcacatcc aacggccctg tgatgcagaa gaaaacactc ggctgggagg
      541 ccttcaccga gacgctgtac cccgctgacg gcggcctgga aggcagaaac gacatggccc
      601 tgaagctcgt gggcgggagc catctgatcg caaacgccaa gaccacatat agatccaaga
      661 aacccgctaa gaacctcaag atgcctggcg tctactatgt ggactacaga ctggaaagaa
      721 tcaaggaggc caacaacgag acctacgtcg agcagcacga ggtggcagtg gccagatact
      781 gcgacctccc tagcaaactg gggcacaagc ttaattaagc ggccgcgact ctagatcata
      841 atcagccata ccacatt
//
